Investigation Of The Correlation Between The Expression And Pathological Characteristics Of P53、p16and P-gp Protein In Gastric Carcinoma

Purpose With gastric cancer tissue microarray to explore the correlation between the expression and clinicopathological characteristics of p53, p16and P-gp Protein in gastric cancer; to provide a new biomarker for diagnosis and prognosis of gastric carcinoma.Methods Tissue microarray was made from121specimens of gastric cancer and39specimens of normal gastric tissues; with immunohistochemical technology, the expression of p53, p16and P-gp protein was detected both in gastric cancer and normal gastric tissues.Results The positive expression rate of p53, p16and P-gp protein in121specimens of gastric cancer was74.4%.19.8%and47.9%, respectively. While0%,76.9%and15.4%in39specimens of normal gastric tissues. The difference was significant, respectively (P<0.001). The results of the correlation between the expression and pathological characteristics of p53, p16and P-gp Protein in gastric cancer:The positive expression rate was75.6%、18.9%and51.1%in90specimens of gastric cancer of male, respectively.71.0%、22.6%and38.7%in31of female, respectively. The difference was without significant (P>0.05), respectively. Positive expression rate of p53, p16and P-gp protein was81.0%,16.7%and45.2%in42cases of age below60, and73.4%,21.5%and49.4%in79cases of age beyond60; the difference was without significant (P>0.05), respectively. The positive expression rate was72.1%,17.6%and51.5%in68specimens of gastric cancer which diameter<5cm, and77.4%,18.9%and45.3%in53of diameter>5cm; the difference was without significant (P>0.05), respectively. The positive expression rate of p53was33.3%in histopathologic grading I,63.3%in grading Ⅱ,81.8%in grading Ⅲ and80.0%in grading Ⅳ. The differences of p53protein expression between grading Ⅱ,Ⅲ,Ⅳ and Ⅰ,Ⅲ,Ⅳ and Ⅱ were significant (P<0.001), respectively. The positive expression rate of p16was66.7%,33.3%,10.4%and20.0%, respectively. Grading Ⅱ, Ⅲ and Ⅳ compared with grading Ⅰ were significant(P<0.001), respectively. Grading Ⅲ and Ⅳ compared with grading Ⅱ were significant(P<0.001, P<0.05), respectively. The positive expression rate of P-gp protein was33.3%,27.3%,55.8%and80.0%, respectively. Grading Ⅲ and Ⅳ compared with grading Ⅰ and Ⅱ were significant(P<0.001), respectively. The positive expression rate in46cases with mucosa and submucosa invasion,6cases of muscular layer invasion, and38cases of serosa and its peripheral invasion, p53protein was51.0%,78.6%and93.1%, respectively. p16protein was30.6%,14.3%and12.1%, respectively. P-gp protein was32.7%,35.7%and64.0%, respectively. The differences between the muscular layer invasion and mucosa&submucosa layer invasion, serosa layer&its peripheral invasion and mucosa&submucosa layer invasion were significant (P<0.001, P<0.01). The positive expression rate of p53, p16and P-gp protein was85.9%,10.9%and52.2%, in92cases with lymph node metastasis.38.0%,48.3%and34.5%in29cases without lymph node metastasis, There were significant differences of p53, p16and P-gp protein positive expression between lymph node metastasis and without lymph node metastasis(P<0.001). The positive expression rate of p53, p16and P-gp protein was86.4%,6.8%and88.6%in44cases distant metastasis,67.5%,27.3%and24.7%in77of cases without distant metastasis, There was difference significant (P<0.01), respectively.Conclusions Our study highlights critical roles for high expression of p53and P-gp protein, low expression p16protein in gastric cancers development, and closely correlated with the histopathological grading, lymph node metastasis and distant metastases of gastric cancer, and suggests that the expression of p53, p16and P-gp protein may be marker in gastric cancer’s diagnosis and prognosis.