The Study Of Mechanism Delay Opacity Of Lens To The Rats Of Diabetic Cataract By3-Aminobenzene

Objective：In this experiment,we utilized PARP(poly ADP-ribosepolymerase) inhibitors3-aminobenzene(3-AB) to intervent the diabetic ratswhich induced by STZ(streptozotocin) mainly,observed the progress of itsdegree of opacity of the lens and detected the relevant indicators,explored themechanism delay opacity of lens to the rats of diabetic cataract by PARPinhibitors.Methods:1, we selected40weight200±20g male Wistar rats, excluded lenslesions through slit lamp inspection.Free drinking water, fast24h after adaptivefeeding3days, extracted9rats as normal control group (group A) radomly,intraperitoneal injection of0.1mol/L citrate buffer (50mg/kg, pH4.5), and therest intraperitoneal injection of1%Stretozocin(STZ) citrate buffer (0.1mol/Lcitrate buffer temporary configuration, pH4.5). Taked venous blood after3days, measured fasting blood sugar is equal or greater than16.7mmol/L fordiabetic rats.2, The diabetic rats be divided into diabetes group (group B) and3-ABinterention group (group C) randomly. Since on the third day after the modeling,group C give3-AB30mg/kg gavage daily, the group A and B group give thesame volume of0.9%N.S gavage every day. Observed and recorded thegeneral situation of the rats and weight in the test, tested fasting blood sugaronce a week.3, Observed and recorded the opactive of the lens groups progress at the slit lamp after dilated by1%amide compound tropicamide eye drops fully, once aweek, the records of method refer to the Foreign Medical OphthalmologyVolume about lens opacities classification. After the administration,2w,4w,8wsacrificed rats in each group respectively. Each group of each take3eye fixedembedding, production of paraffin sections for immunohistochemical,SP wasdetected the expression of MMP-2(matrix metalloproteinase-2)and bFGF(basicfibroblast growth factor)in lens epithelial cells; The other three were detectedthe activity of glutathione peroxidase(GSH-PX) and superoxide dismutase(SOD) and the level of malondialdehyde (MDA) and advanced glycation endproducts(AGE)after separate lens.Results:1, Selected9as normal control group (group A) rats grew well, usedSTZ made diabetic rats model, finally only18inclued in the experiment, and allappeared the typical diabetes performance of a little over.2,2,4,8weeks in the experiment,the blood glucose of B, C group above groupA, difference was significant (P <0.01), but compared the group B and C, theblood glucose without difference (P>0.05).3, During the whole experiment, A group always keep the lens transparent, andwith the experiment progresses, group B, C all began to varying degrees of lenscloudy in the third week. A, B and C three groups have the degree of lenscloudy were significantly in the fourth and eighth week(P <0.01), and heavythan group A (P <0.01).4, Immunohistochemical results:almost no expression of MMP-2in group A,but as the experiment progresses,the expression for B, C groups increased gradually, and group B is stronger than group C (P <0.01);the pression of bFGFin A, B and C were positive,there were significant difference between A,B andC group(P <0.01), and the pression enhanced as the experiment progresses in B,C group, but no difference between B and C group(P>0.05).5, The detection of the oxidation index: The GSH-px and SOD activities of Agroup are higher than B, C two groups (P <0.01), however,each time group Bare lower than group C (P <0.01, P <0.05); The content of MDA increase inB,and C group, compared with A (P <0.01), and B group higher than group C(P <0.05).6, The content of AGE in group A were lower than group B and C;2,4weeks,group B higher than group C (P <0.05), but no difference at8weeks(P>0.05).Conclusion:1, PARP inhibitors,3-AB has aprotective effect on the diabetic ratslens can delay diabetic cataract development.2, The opacity of lens epithelial cells because of oxidative damage andnon-enzyme glycation level result from high sugar can be reduced by PARPinhibitors3-AB.3, PARP inhibitors3-AB inhibit the expression of MMP-2the lens epithelialcells and reduce the extracellular matrix degradation,so as to achieve the role ofdelayed diabetes cataract.3, bFGF involved in the development of diabetic cataract.