Association Of HLA-DRB1Alleles With Anti-neutrophil Cytoplasmatic Antibodies In Han And Uygur Patients With Ulcerative Colitis

Objective: To determine the association between the HLA-DRB1alleles andanti-neutrophil cytoplasmatic antibodies (ANCA) positive in Han and Uygur patients withulcerative colitis (UC). Methods:62Uygur and58Han patients with UC, confirmed bybiopsy and followed up for half a year, were studied. Meanwhile,188Uygur and184Hannormal persons were enrolled as healthy controls. Polymerase chain reaction sequencebased typing (PCR-SBT), provides the highest resolution level, were used for HLA-DRB1typing. Serum ANCA was detected with indirect immunofluorescence assay (IIFA).Results: ANCA was detected in thirty-three of Sixty-two (53.2%) Uygur patients with UC,twenty of fifty-five (34.5%) Han patients with UC. It showed that the positive rate inUygur patients with UC was significantly higher than in Han patients with UC (χ~2=4.269,P=0.045). In Uygur people, ANCA positive UC patients had a significantly increasedfrequency of HLA-DRB1*13compared with ANCA negative controls (P_c=0.014, OR=16,CI95%=2.8919～88.5242), furthermore, HLA-DRB1*13was significantly increased infrequency in UC patients with pancolitis and ANCA positivity (P_c=0.035, OR=19.5,CI95%=2.7865～136.4606). But in Han patients, HLA-DRB1alleles showed nosignificant differences between ANCA positive UC patients and ANCA negative controls,also, no significant differences were found between ANCA positive patients, HLA-DRalleles and other clinical features such as severity, extension. Conclusions: TheHLA-DRB1*13is not only associated with ANCA positive UC patients of Uygur peoplein Xinjiang province, but also associated with ANCA in patients with pancolitis. But thisassociation is not found in Han patients.