Research On The Effect Of Astragaloside â…£ Prevents Cartilage Degeneration Of People With Knee Osteoarthritis

Objectives:To investigate the effect of AstragalosideIV adjust MMP-1、MMP-3mRNA expression in chondrocyte of People with knee osteoarthritis.Methods:Recipe the cartilage of People with knee osteoarthritis after operation, to carry out cell culture, when cells are more enough, go down to the future generation and use the third generation cells, stain them with HE、jia ben an lan, fluorescein stain with Ⅱ-collagen to checkout they are the cartilage cells. fluorescein stain with MMP-1and MMP-3to checkout they are the degeneration cartilage cells.Then the third generation cells are to divide equally six group(group A:cartilage cells+control group of DMEM; group B:cartilage cells+concentration of AstragalosideⅣ is25mmol/L; group C:cartilage cells+concentration of AstragalosideⅣ is50mmol/L; group D:cartilage cells+concentration of AstragalosideⅣ is75mmol/L; group E: cartilage cells+concentration of AstragalosideⅣ is100mmol/L; group F:cartilage cells+concentration of AstragalosideⅣ is500mmol/L), at4h、8h、24h、48h、72h, Total RNA isolated from these samples was used for real-time PCR of matrix metalloproteinase-1(MMP-1), matrix metalloproteinase-3(MMP-3).Results:Culture’s cells are checkouted cartilage cells.Real-time PCR showed concentration of AstragalosideⅣ at(25mmol/L-100mmol/L) downregulated mRNA expression of MMP1, MMP3.Conclusion:Astragaloside Ⅳ can prevents Cartilage degeneration of People with knee osteoarthritis.Conclusions:Astragaloside Ⅳ can Prevents Cartilage degeneration of People with knee osteoarthritis.