| Objectives:To explore the protective effects of local subarachnoid infusion with Hydrogen Saline (HS) on spinal cord injury (SCI) at rats, and analyze the probable mechanism of its protective treatment role.Methods:Seventy-five rats, weighted200-250g, were randomly divided as three groups:group A (n=25), group B (n=25), each rat received injection with the same amount of Hydrogen Saline, and group C (n=25), each rat received injection of normal saline (NS).The rats of all group were assigned toA1(6h),A2(24h),A3(48h),A4(1w),A5(2w); B1(6h), B2(24h), B3(48h), B4(lw), B5(2w) groupand C1(6h),C2(24h), C3(48h), C4(1w), C5(2w) group at6hours,24hours,48hours,1week,2weeks after SCI, respectively. All therats of B、C group received injection into the subarachnoid space by the catheter, after establishing model of spinal cord injury by the improved Allen’s collision method, and all the rats of A group were treated with the vertebral plate opened and Subarachnoid catheter. At the corresponding time, the BBB score of all ratswere enforced, the content of Spinal cord tissue were detected withHE staining for pathological observation, and detected the expression the expressions level of CGRP> Caspase-3with immunohis-tochemistry. Before all rats were killed at each time point, were also exsanguinated for detection of malondialdehyde and superoxide dismutase.Results:1. Assessment of Movement (BBB score):The score of all subgroup in A group is21points; After24hours,48hours,1week and2weeks, the BBB score of B group was higher than that of C group at appropriate times (P<0.05).2. HE staining:The cell morphology were intact, arranged com pactly, and the nuclei were hyperchromatic in the A group.Compar ed with group B1, the inflammatory cell infiltration of group C1wa s more evident; Compared with group C2, the inflammatory cell inf iltration of group B2was significantly milder; compared with group B3, the inflammatory cell infiltrationand and Neuron atrophy of gro up C3was more evident; compared with group C4, the Neuron atr ophy of group B4was significantly milder; and the connective tissu e formation of group C5was significantly more than group B5.3. CGRP OD:After24hours,48hours,1week and2weeks, the mean optical density of B group was higher than that of C group at appropriate times (P<0.05).4. The biological activity assay of MDA:After24hours,48hours,1week and2weeks, the level of malonyl dialdehyde of C group was higher than that of B group at appropriate times (P<0.05).5. The biological activity assay of SOD:After24hours,48hours,lweek and2weeks, the level of superoxide dismutase of B group was higher than that of C group at appropriate times (P<0.05).6. The immunohistochemistry of Caspase-3:After24hours,48ho urs,lweek and2weeks, the numbers of the positive expression neu rons of C group was higher than that of B group at appropriate ti mes (P<0.05),and that of B group was higher than that of A group.Conclusion:1. Hydrogen-rich saline can reduce the inflammatory status and oxidative stress early after spinal cord injury, and enhance the anti oxygenexpected in later development stages, so as to inhibit the ne ural apoptosis and improve the neural function.2. Through restrainting the decline of CGRP early after SCI, a nd enhancing CGRP expression at in-depth at a later stage in spina1cord anterior horn motoneurons, hydrogen-rich saline can protect s pinal nerve cell. |
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