| Objective: This experiment applies HE,Western Blot,ELISA and other methods on mouse cerebral hemorrhage models to explore whether the Chinese traditional medicine decoction for removing blood stasis and replenishing the essence and replenishing the marrow can improve the NF-?B inflammatory pathway in mice with cerebral hemorrhage by regulating Nrf2.Improve secondary injury after cerebral hemorrhage.To explore whether Nrf2 is a target for the treatment of cerebral hemorrhage by breaking blood and removing blood stasis and filling the marrow.Methods: 1.Select wild-type C57 male mice and Nrf2-/-male mice,weighing 22g-25 g,and randomly divided into sham operation group(group 1),Nrf2-/-sham operation group(group 2),and cerebral hemorrhage Model group(group 3),Nrf2-/-cerebral hemorrhage model group(group 4),hemorrhagic stroke formula group(group 5),Nrf2-/-hemorrhagic stroke formula group(group 6),25 in each group.A stable cerebral hemorrhage model was established by autologous tail artery blood injection.2.Observe the neurological deficits,brain water content and pathological conditions of the brain tissue around the hematoma by neurobehavioral score,wet and dry weight method,and HE staining method.3.ELISA was used to detect the changes of IL-1,IL-6 and TNF-? expression in the brain tissue around the hematoma.4.Western Blot method was used to detect the changes of NF-?B and Nrf2 protein expression.Results:1.The Longa score of all experimental mice before surgery was 0.There were no neurological dysfunction and normal activities in the sham operation group 1 and 2.The dysfunctions such as loss of consciousness,hemiplegia,and rotation occurred in the mice of the 3,4 model group and the 5 and 6 formula groups.The behavioral scores of the three groups were significantly increased,compared with the sham operation group of the 1 group,there was a significant difference(P<0.05);the behavioral scores of the 5 group prescription group were significantly increased,and there were significant differences compared with the sham operation group of the 1 group(P <0.05);the behavioral scores of the 3 model groupswere different from those of the 5 prescription group;there was no difference in the behavioral scores of the 3 model groups and the 4 model group;The scores increased significantly,compared with the sham operation group of the two groups,there was a significant difference(P<0.05);the behavioral scores of the 6 group prescription group increased significantly,and there was a significant difference(P<0.05),compared with the sham operation group of the two groups;The behavioral scores of the 4 model groups were different from those of the 6 prescription formula groups;there were significant differences in the behavioral scores of the 5 prescription groups and the 6 prescription groups(P <0.05).2.Compared with that,there was a significant difference(P<0.05).The brain water content of the 5 group prescription group was significantly different from that of the sham operation group(P <0.05);the brain water content of the 3 group model group was 5 There was a difference in brain water content in the prescription group;there was no significant difference in brain water content between the 3 model group and the 4 model group;compared with the brain water content in the 4 group and the sham operation group,there were Significant difference(P<0.05);The brain water content of the 6 group prescription group was significantly different from the brain water content of the sham operation group(P<0.05);the brain water content of the 4 group model group was compared with the 6group prescription water group.There are differences;there is a significant difference between the 5 groups and the 6 groups(P<0.05).3.1.2 Sham operation group: 3 days after intracerebral hemorrhage,the brain structure of the rat is complete,the nuclei are clear,the nerve cells are full and dense,the shape is normal,the arrangement is neat,and there is no edema.4.The expression levels of IL-1,IL-6 and TNF-? in the three groups were significantly different from those in the sham operation group(P<0.05).There were significant differences(P<0.05);there were differences between the three model groups and the five prescription groups;there were no significant differences between the three groups and the four groups.There were significant differences between the 4 model groups and the2 sham operation groups(P<0.05),and there was a significant difference between the 6group prescription groups and the 2 sham operation groups(P<0.05);the 4 group model groups Compared with the 6 group prescription group,there are differences;the 5 group has significant differences compared with the 6 group(P<0.05).The results suggest that the treatment of cerebral hemorrhage by smashing blood and removing blood stasis and filling the marrow can play a role by reducing the levels of IL-1,IL-6 and TNF-?.5.3 days after intracerebral hemorrhage,the protein expression level of Nrf2,the Nrf2 content of the 3 model groups was significantly increased compared with the Nrf2 content of the 1 sham operation group,and the expression of the 5 prescription groups was reduced.The protein expression level of NF-?B,the content of NF-?B in the three model groups was significantly reduced compared with the content of NF-? B in the sham operation group,and the content of NF-?B in the prescription group 5 was compared with the content of NF-?B in the model group 3 Significantly increased,the above results show that the traditional Chinese medicine decoction of breaking blood and removing blood stasis,filling the essence and replenishing the marrow can regulate the NF-?B signaling pathway by regulating Nrf2.Conclusion: 1.The method of breaking blood and removing blood stasis and filling the marrow can promote the expression of Nrf2 and reduce the expression of NF-?B,thereby reducing secondary damage and exerting the role of brain protection.2.Decoction,blood-stasis-removing,essence-filling and marrow-filling Chinese medicine decoction may improve the NF-?B inflammatory pathway in mice with cerebral hemorrhage by regulating Nrf2. |
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