Influence Of Epigallocatechin-3-gallate On Chemokines Expression Induced By Scald In Mice Skin

The wound healing is a complex pathophysiology process involving in inflammatory response, newtissue formation and remodeling of tissues. During the inflammatory response, the cytokines andchemokines, which are secreted by cells residued in tissue, recruit the neutrophils, macrophages and otherimmune cells into the wound site to join the inflammatory response, and provide immune effector cells andcell growth promoting factors for the process of the wound healing. The chemokines play an important rolein the inflammatory responses as well as the formation of the new organizations and the reconstruction ofthe tissue.Epigallocatechin-3-gallate(EGCG) is an active ingredient of green tea. And it has anti-inflammatory,antioxidant, antiradiation, anti-aging, antibacterial, inhibiting tumor cell proliferation, inducing tumor cellcycle stasis, initiating apoptosis and other biological effects.Preliminary studies showed that EGCG could inhibit the IL-1β, TNF-α and MMP-1gene expressionduring the wound healing, reduce the levels of IL-1β, TNF-α and MMP-1, and promote the expression ofTIMP-1gene. In this thesis, the author researched the spatial and temporal expression of monocytechemoattractant protein-1(monocyte chemoattractant protein-1, MCP-1) and macrophage inflammatoryprotein-2(macrophage inflammatory protein-2, MIP-2) during the wound healing in mice burn model withreal time PCR and ELISA, and further explored the influence of EGCG on the expression of MCP-1andMIP-2gene. The results showed that the deepⅡdegree mice scald repair process: the expression of MCP-1mRNA levels and MCP-1protein levels in the regional skin first increased and then decreased, and MIP-2mRNA levels and MIP-2protein levels expression firstly increased and then decreased and then increasedand decreased again. After skin burn parts were coated with0.2mg/g of EGCG, the expression levels of theMCP-1and MIP-2gene decreased in the wound tissue. It suggested that0.2mg/g EGCG was able toinhibit the MCP-1and MIP-2gene expression in the site of injury, and could promote the healing of micewound skin.