Study On The Correlation Between ANXA₁and LIVIN Expressed In Renal Cell Carcinoma （RCC）；iud The Clinical Significance

Objective：By detecting on the difference and relationship of annexin a1（ANXA₁）, LIVINexpressed in RCC and their relationship with RCC clinicopatho-logical factors（clinical stage, distant metastasis, lymph node, breaking the capsule and no,histological grade, tumor location, tumor diameter, age and gender）; Explor-ingpotential role of ANXA₁in the development of the RCC and relationship with LIVINthat is proteins invasion, malignancy degree of relevant of the RCC.These isconducive to further study the role of the molecular mechanisms of ANXA₁. It willprovide a theoretical basis and the ideal target for for further genetic diagnosis、 risktransfer, radiotherapy and chemotherapy, interventional therapy and target therapy.Methods：Collected paraffin blocks of50patients which have been diagnosed patholo-gically RCC after surgical resection from December2006to July2011at the FirstAffiliated Hospital of Nanchang University. Collected paraffin blocks of10patientsnormal renal tissue from no-RCC patient. Each patient’s tumor location, tumordiameter, Histological grade, depth of invasion, lymph node, distant metastasis andclinical stage were confirmed pathologically after surgical resection. All patients weregrouped by clinical and pathological factors （clinical stage, distant metastasis, lymphnode metastasis, breakthrough coated or not, histological grade, tumor location, tumorsize, age and sex）. Nobody have received any tumor therapy （chemotherapy、radiotherapy、 interventional therapy or target therapy） in preoperative. Eachorganization selected paraffin cut into3pieces and each piece thickness is4μm. Wedetected the expression of ANXA₁and LIVIN of RCC and normal gastric tissuesamples by Two-step immunohistochemistry and analyzed its differences and therelationship with clinical pathological factors （clinical stage, distant metastasis,lymph node metastasis, breakthrough coated or not, histological grade, tumor location,tumor size, age and gender）. Data processing and analysis using the SPSS15.0 statistical software, ANXA₁and LIVIN expression in normal kidney tissue and RCCif there were differences、 the relationship between expression andclinicopathologicalfactors in RCC using the card square test, ANXA₁and LIVIN expression in RCC andnormal kidney tissue is related to the column associated correlation analysis, p≤0.05is statistically significant and testing standards to take sides.Results：1. ANXA₁, LIVIN in RCC and normal renal tissue IHC staining results（1） In RCC and normal kidney cells ANXA₁located in the cytoplasm and thenucleus, The positive cells appeared brown granules in the nucleus and brown-yellowgranules in cytoplasm, while the negative cells show a small amount of yellowparticles or no particles in the cytoplasm, the nucleus is not stained. Part of the mese-nchymal cells are also staining.（2） In RCC and normal kidney cells LIVIN located in the cytoplasm and thenucleus, The positive cells appeared brown granules in the nucleus and brown-yellowgranules in cytoplasm, while the negative cells show a small amount of yellow parti-cles or no particles in the cytoplasm, the nucleus is not stained. Part of the mesenc-hymal cells are also staining.2. The relationship of the expression of ANXA₁in RCC and normal renal tissuewith clinicopathological factors.ANXA₁0cases expressed in10cases of normal renal tissue is shown in Table3.3,the positive rate of0%（0/10）;35cases of ANXA₁expression in50cases of renal cellcarcinoma, the positive rate was70%（35/50）, The former was significantly lowerthan the latter, P value <0.001,the expression of both ANXA₁significant difference.The expression of ANXA₁in RCC tissue wasn’t associated with patient’s gender, age,tumor size, tumor location （p>0.05） and associated with cell different-iation,invasion depth, lymph node metastasis,distant metastasis and clinical stage （p<0.05）.3. The relationship between the LIVIN expression in RCC and normal renaltissue and clinicopathological factorsLIVIN0cases expressed in10cases of normal renal tissue is shown in Table2, the positive rate of0%（0/10）;27cases of LIVIN expression in50cases of renal cellcarcinoma, the positive rate was54%（27/50）. The former was significantly lowerthan the latter, P value=0.002, the expression of both LIVIN significant difference.The expression of LIVIN in RCC tissue wasn’t associated with patient’s gender, age,tumor size, cell differentiation, tumor location （p>0.05） and associated withinvasion depth, lymph node metastasis,distant metastasis and clinical stage （p <0.05）.4. The correlation between the different expression of ANXA₁, LIVIN in RCC tissueThe coefficient associated column is0.096between the expression of ANXA₁and LIVIN in RCC tissue （r=0.096）, no significant correlation （p=0.496）.Conclusions：1. ANXA₁is over expression in RCC and is no expression in normal renal tissue.The expression of ANXA₁in RCC tissue wasn’t associated with patient’s gender, age,tumor size, tumor location and associated with cell differentiation, invasion depth,lymph node metastasis, distant metastasis and clinical stage.2. LIVIN is over expression in RCC and is no expression in normal renaltissue.The expression of LIVIN in RCC tissue wasn’t associated with patient’s gender,age, tumor size, cell differentiation, tumor location and associated with invasiondepth, lymph node metastasis,distant metastasis and clinical stage.3.ANXA₁and LIVIN over-expression maybe no significant correlation.