| ObjectiveIntended by immunohistochemistry, perspective electron microscopy and othermethods of vulnerability District glial scar formation after diffuse axonal injury in therat temporal and spatial distribution disciplineMethods1.A rat model of DAI established by an experimental device independently designed(Patent No.: ZL200920067463.4) for inducing simultaneous linear and angularaccelerations. Then rats were sacrificed for hematoxylin eosin (HE) staining,immunohistochemical staining to evaluate DAI and appreciate the ultramicroscopicchanges longitudinally.2. Brainstem, corpus callosum, internal capsule, white matter and other vulnerableareas by immunohistochemical staining axis shrinking ball formation, neurofilamenttangles formation of myelin disintegration, the IOD values of each vulnerable areathan the control group were significantly higher,statistically significant; after injury,astrocytes in each ROI activation number3days significantly increased7daysmaximum, continuing a month.3. DAI vulnerable area activated microglia IOD values than the control group wasstatistically significant at all time points. Microglia activation3days after injurysignificantly increased7days to achieve the highest value and gradually reduced, andcontinued until1month after injury.4. DAI after injury around the neurofilament gathered a large number of activatedastrocytes and microglia, and vulnerability of the area there are "micro-scars" impedenerve regeneration.Results1.HE staining to non-occupying micro-hemorrhagic focus; immunohistochemicalstaining found axis shrinking ball formation, neurofilament tangles form. 2. The number of DAI activation of vulnerability District astrocytes3dayssignificantly increased7days maximum, and continue until one month after injury.ConclusionsAfter the injury of rats with DAI vulnerable microenvironment in differentdegrees of micro-glial scar formation, and have a certain amount of time and spacediscipline. |
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