Expression And Clinical Significance Of HGF And C-Met Receptor In Osteosarcoma

Objective: Studies suggest HGF/c-Met signalling is associated withthe tumorigenesis and progression of various tumors. The study was toinvestigate the expression of HGF/c-Met in osteosarcoma and itscorrelation with clinicopathology.Methods: The expression of HGF/c-Met of30osteosarcomas，15callus and17multiple osteochondromatosises was detected byimmunohistochemistry. The experimental data were statistically analyzedby SPSS l9.0.Results:1.HGF express in osteosarcoma cell cytoplasm and thepositive expressive rate in osteosarcoma was40.0%（12/30）. In callus HGFexpress in interstitial multinucleated giant cell cytoplasm，the positiveexpressive rate in callus was13.3%（2/15）.Negative expression wasdetected in multiple osteochondromatosis. There were statistical significantdifference between osteosarcoma and multiple osteochondromatosis（P<0.001）. The HGF expression in osteosarcoma increases along with the advances of Ennecking′s stage (P<0.05)，The expression of HGF in groupswith and without metastasis are88.9％(8／9) and19%（4/21）,respectively.There is statistical significance between the two groups(P=0.001).2. c-Metexpress in the cytoplasm of parenchymal cells. the positive expressive ratewas73.3%（22/30）in osteosarcoma,40.0%（6/15）in callus and5.9%（1/17）in multiple osteochondromatosis. There were statistical significantdifference between the three groups (P<0.05). The expression of c-Met ingroups with and without metastasis are100%（9/9） and61.9%（13/21）,There is statistical significance between the two groups(P=0.001).3.thecoexpression of HGF and c-Met in group with metastasis is66.7％(6／9)，higher than non-metastasis group14.3%（3/21）。Conclusions: The expression of HGF and c-Met increase inosteosarcoma. The expression of HGF and c-Met in osteosarcoma’smetastasis play an importantroel，and HGF expression correlated withmalignant degree of osteosarcoma． HGF and c-Met co-express inosteosarcoma cell cytoplasm,suggest that HGF/c-Met signalling promoteosteosarcoma’s metastasis through autocrine loop.