Study Of Effect Of Meloxicam On Hep-2Cell Line In Laryngeal Carcinoma

ObjectiveTo research the effect of Meloxicam on cultured laryngeal carcinoma Hep-2cellline apoptosis, growth cycle and the potential mechanism.MethodsThe laryngeal carcinoma Hep-2cells were cultured in vitro, and0,0.4,0.8,1.2and1.6mol/L Meloxicam were added. Cell growth and cell viability was analyzed at24,48and72h. Flow cytometry was used to detect cells apoptosis and the cell cycle,the mitochondrial transmembrane potentials was also assessed. The protein expressionwas determined by Westrn blot test.Results1. Different concentrations of Meloxicam inhibit Hep-2cells proliferation and thecell vitality in dose-dependent manner from0~24h；2. Flow cytometry showed that the apoptosis cells increased with the increase ofMeloxicam concentration, the differences were significant (P<0.05);3. Flow cytometry showed Meloxicam could change the cell cycle in differentdosage, as the concentration increased, the G1cells were also increased.4. Flow cytometry showed The mitochondria membrance potential declinedobviously with the increasing of Meloxicam concentration. In addition, the samephenomenon was also appeared with prolonged drug interactions.5. Western blot showed that Meloxicam could down-regulated bcl-2levels inHep-2cells.Conclusion1. Meloxicam significantly inhibits Hep-2cells growth and induces themapoptosis in dose-and time-dependent manner； 2. Meloxicam inhibits the growth and induces apoptosis was mediated bymitochondrial pathways and was associated with down-regulated bcl-2expression.