The Expression And Significance Of High Mobility Group Box1Protein In The Balf Of COPD Patient

Research background：chronic obstructive pulmonary disease(COPD) is a diseasethat the etiological factor and pathogenesis are not understood entirely，with highprevalence rate and mortality rate.It mainly involve the lung as well as whole system.The characteristic pathology locating in center and peripheral airways, lung parenchyma, pulmonary vasculature contain chronic inflammation that displayincremental inflammatory cell in lung and airway remodeling induced repeating lesionand restore. The pathogenesis of COPD is not very clear.The current view to thepathogenesis of COPD that the disbalance of protease-antiprotease and the disbalanceof oxidation-antioxydation as well as the chronic airway inflammation commonlyinduce the pathological change.inflammation that involve multiple inflammatory cells,airway structural cells and inflammatory mediators is in center of pathogenesis. Highmobility group box-1protein is a highly conserved prevalent nuclear protein whichparticipates in DNA transcription, replication, repairment and inflammatoryreactions.It is studied that HMGB1acts as a leading role in the pathogenesis ofCOPD.One hand,the airway inflammatory cells such as macrophagocyte, monocytesand airway structural cells such as epithelial cells, smooth muscle cells express andsecret grossly HMGB1,on the other hand,HMGB1can promote release ofinflammatory cytokines that advance airway inflammation. In the research we abstractthe bronchoalveolar lavage fluid(BALF) of patient suffering COPD and measure andanalysis the content of HMGB1and other inflammatory cytokine in BALF so as toexplore the effection of HMGB1to the COPD.Objective: By evaluate HMGB1in the BALF of patient suffering COPD, we exploreinitially the effect of HMGB1to the characteristic pathology of COPD: airwayinflammation.Methods:The objects that were grouped patient group and control group were alveolarwashed respectively and the bronchoalveolar lavage fluid was extracted. Analysing thecell counting and classification of two group BLAF and assaying the HMGB1, IL-1β，IL-8and TNF-αin BLAF with ELISA assay. The correlation between HMGB1andother inflammatory factor as well as severity of desease was evaluated and analysed. Results:The total cell counting, mononuclear macrophage counting, neutrophilicgranulocyte counting in BLAF of control group were respectively0.74（0.23-1.46）×10~9/L，89.2（34.1-96.6）%，8.0（1.3-17.2）%.It were4.16（0.94-9.73）×109/L，58.3（25.2-74.0）%，33.4(19.6-57.9)%in BALF of patient group. The total cellcounting, neutrophilic granulocyte counting in BLAF of patient group weresignificantly higher than control group (P<0.05). Reversely,the former is lowerthan thelatter in mononuclear macrophage counting(P<0.05). The lymphocyte counting inBALF of patient group and control group was respectively2.4（3.6-8.6）%、2.7(4.3-9.7)%,the two group have no significant difference(P>0.05).The concentrationof HMGB1(ug/L), IL-1β（ng/L），TNF-α（ng/L）,IL-8（ng/L） in BALF of patient groupand control group were respectively8.95(0.01-32.00)，3.62(0.01-11.30)，0.97（0.35-14.50），3.45(0.01-19.96)/2.35(0.01-29.00)，0.45(0.01-5.56)，0.56（0.01-3.50），3.16(0.01-19.54)，the former is significant higher than the latter（P<0.05）.Theconcentration of HMGB1in BALF of patient group has linear correlation with theconcentration of IL-1β、TNF-α（r=0.685,R=0.624，P <0.05）.In patient group,theconcentration of HMGB1in BALF extracted from middle and severe patient issignificantly higher than that from gently patient（P<0.05）.Conclusions:The expression of HMGB1in BALF extracted from COPD patient issignificantly increased and correlate with the severity of disease.The concentration ofHMGB1in BALF extracted from COPD patient has significant correlation with that ofinflammatory factor such as IL-1β、TNF-α.