The Pharmaceutical Research Of Armillariella Capsule

Hepatitis is an inflammation of the liver, and as a high incidence of transmitteddiseases, hepatitis is a serious threat to our health and daily life. The common hepatitiswe referred to is caused by virus, which is divided into six different types of rivals,each of this Piru can cause liver cell swelling, dispread and make hepatitis be one ofthe great infectious diseases. Otherwise, a lot of anti-hepatitis virus drugs bored.especially the accumulation of long-term clinical practice of Traditional ChineseMedicine, prevention of hepatitis laid the foundation for its drug research.Armillariella tabescences is a fungal Door Basidiomycetes, Agaric-ales, MushroomDecision, false dense cycle of Armillaria spp the bacteria Armilla tabescences (Scop.exFr) Sing. Its sweet cool with the heat and dampness, and have the effect of relieving QiStagnancy in Liver, play a significant role in prevention and treatment of hepatitis. asthe Traditional Chinese Medicine which is wisdom from folk and has its independentintellectual property rights of a fungus, Armillariella tabescences is the viable medicineas a treatment of acute and chronic hepatitis, whose main component is itspolysaccharides.The main contents were divided into some sections as follows: 1. Studies on the extraction technology of the Armillariella polysaccharideUse the orthogonal experimental method to optimize the extraction process on theArmillariella bacteria active ingredient, to determine the production process of aqueousextract Armillariella polysaccharide in the optimum extraction process was1/4timesof water, extracted for2times, each time for150min, the temperature was100℃. Andto aim the adding alcohol’s times and the Concentration, to determine the alcoholprecipitation method to extract polysaccharides concentration of100%percentageethanol, three ethanol sedimentation, before two have joined the10ml, adding6ml atthe third time.2. Study on quality standard of Armillariella tabescences water extract1) Study on sulfuric acid-phenol method of measurement of ArmillapolysaccharideBasis on the maximum absorbance values, the phenol concentration and addingamount of sulfuric acid from the polysaccharide, selected the most suitable methodsfor determination of polysaccharides content of sulfuric acid and the concentration ofphenol. To determine the wavelength is490nm, sulfuric acid is added to5ml; phenol’sconcentration is3%adding1.0ml.2) The determination in the aqueous extract of polypeptide armillarisinBy researching literatures to comparing a variety of determination of polypeptidemethods, choose to use Folin-phenol reagent method to measure the content ofpeptides in armillarisin extraction, determination conditions: add a liquid phenol solution of1.0ml into the sample solution, put it at a normal atmospheric temperaturefor10min, then join the Fo-lin phenol reagent B liquid4.0mL, strain keep warming at55℃for5minutes after straining. The absorbance number is650nm.3) The determination of armillarisin in the aqueous extractThrough to contrast about extraction method、high performance liquid chromatographyconditions of armillarisin. determined the extraction method for armillarisin is: first,stratificational with4ml ethanol, and then joining15ml ether, extracting for three times,use the combined filtrate as the sample. The chromatography conditions are: flow rateof1ml/min, column temperature30℃, a methanol mobile phase:0.1mol/L acetic acid=50:50, the detection wavelength was366nm.3. Drying method and material selection proportioning of the water extract ofArmillariella tabescencesBased on the water extract of Armillariella tabescences drying method comparison andscreening of materials ratio, determined the drying method for water steam dry,materials for starch, dextrin and when the ration is drug: Starch: dextrin=75:18:7, theArmillariella power have the best molding and the moisture absorption is minimum.