| ObjectiveTo investigate the expression and variation of neurokinin A in the dental pulp of the first maxillary molar following the establishment of animal model.Methods30adult male SD rats were divided into6groups randomly,each group had5rats. Giving a mesial tensile force of about50g to the first right maxillary molar, the rats were forced at0d,1d,3d,5d,7d,14d.Tissue blot containing the molar and its surrounding alveolar bone were obtained; neurokinin A immunohistochemistry staining was applied to measure after fixing. The change of the neurokinin A expression in the dental pulp of first maxillary molar and analyzed by average gray values analysis and Statistical analysis of variance and the q test.Results(1) The amount and strength of neurokinin A in the molar pulp of rat were gradually increased after tooth movement,reached the peak after3days,then decreased and came back normally after14days.(2) The average gray values of neurokinin A in the molar pulp of rat were gradually decreased, reached the minimum at3d,then increased and came back normally at14d. Compared the average gray values of the group of strength1,5days with the control group (the group of strength0day), the difference had statistics significance (P<0.05). Compared the average gray values of the group of strength3days with the control group, the difference had statistics significance (P<0.01). Compared the average gray values of the group of strength7,14days with the control group, the difference did not have statistics significance (P>0.05) Conclusions(1) The rat tooth movement stimulates the pulp nerves to release neurokinin A and cause the variation of neurokinin A in the rat tooth pulp.(2) The increase of neurokinin A in dental pulp may cause the pain in the orthodontic treatment early. |
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