To Study The Influence Of Laparoscopic And Open Radical Operation Of Colorectal Carcinoma On Hematogenous Micrometastasis By The Combinative Detection Of GCC MRNA And HTERT MRNA

Objective:To study the expression and significance of GCC mRNA and hTERT mRNA in the peripheral blood samples by laparoscopic and open radical operation of colorectal carcinoma,and discuss the influence of laparoscopic and open radical operation of colorectal carcinoma on hematogenous micrometastasis and the application value of laparoscopic surgery.Methods:The51colorectal cancer patients were devided into open radical operation group(28cases) and laparoscopic group(23cases) at random.Using reverse transcriptase-polymerase chain reaction(RT-PCR) to detect the expression of GCC mRNA and hTERT mRNA in the peripheral blood samples by laparoscopic and open radical operation of colorectal carcinoma patients preoperatively and postoperatively.Results:1、 Both open group and laparoscopic group had the expression of GCC mRNA and hTERT mRNA in the peripheral blood.The positive rate of GCC mRNA and hTERT mRNA were related to the TNM staging and the degree of differentiation,but irrelevant to the patients’age and gender.2、 The postoperative positive rate of GCC mRNA、 hTERT mRNA and positive rate of combinative detection were significant higher than preoperative both in laparoscopic and open radical operation(P<0.05).the positive rate of GCC mRNA、 hTERT mRNA and the positive rate of combinative detection had no significant difference between the laparoscopic and open radical operation of colorectal cancer either preoperatively or postoperatively(P>0.05).3、 To combinative detect the expression of GCC mRNA and hTERT mRNA didn’t improve the positive rate,the difference was non significant(P>0.05).Conclusion:1、 Both laparoscopic and open radical operation of colorectal carcinoma could increase the possibility of hematogenous micrometastasis.2、 Compare with open radical operation of colorectal carcinoma,the laparoscopic radical operation did not increase the risk of hematogenous micrometastasis.