The Experimental Study On The Early Healing Process Of Rabbit Bile Duct Injury With Different Suture Modes

Objective: A model of trauma-repair of common bile duct with continuous sutureand interrupted suture respectively in the rabbit was made. To investigate the earlyhealing process of common bile duct injury with different suture modes. Comparewhether there are differences on bile duct healing after two different suture modes onrabbits.Methods: Thirty-five rabbits were randomly divide into three groups: control group(n=5), continuous suture group (n=15) and interrupted suture group (n=15). Thesamples of the control group were got from the common bile ducts of the fivehealthy rabbits. Two experimental groups were randomly divided into three groupsrespectively, each group was sacrificed on1stweek,2ndweek and4thweek afteroperation respectively. Excised samples were examined for thickness of bile ductwall、 inner girth of bile duct. Intensity of expression of α-smooth muscleactin(α-SMA) and proliferating cell nuclear antigen(PCNA) were studied withimmunohistochemical SP staining in samples. The expression of α-SMA、PCNA andmicrovessel density(MVD) were detected by image analysis software. All resultswere analyzed in statistics finally. Results: Wall thickness and inner girth of bile duct were significantly differentbetween two experimental groups and control group (p<0.05). Wall thickness andinner girth of bile duct were significantly different between continuous suture groupand interrupted suture group on week4after operation (p<0.05). α-SMA and PCNAwere expressed higher in two experimental groups than control group (p<0.05).α-SMA and PCNA were expressed higher in continuous suture groups thaninterrupted suture group (p<0.05). MVD was higher in two experimental groups thancontrol groups (p<0.05).MVD was higher in continuous suture groups thaninterrupted suture group (p<0.05).Conclusions: The repaired bile duct was narrower in continuous suture group thaninterrupted suture group in the early healing process. The number of myofibroblastwas larger in continuous suture group than interrupted suture group. The hypoxia ofbile duct was more serious in continuous suture group than interrupted suture group.The repaired bile duct with continuous suture may be more prone to developstenosis.