Study On The Exposure Levels Of Phthalates In Pregnant Women And Its Influence Factors

ObjectiveThe Objective of this study were detected the concentration of5phthalates metabolites in pregnant women urine, including MMP, MEP, MBP, MBzP and MEHP, appraised the exposure level of PAEs and analyzing the possible factors influencing pregnancy exposure to PAEs.MethodsIn this study we recruited one hundred and ninety pregnant women in Tianjin between March2011and July2011. At the time of recruitment, participators were asked to complete a questionnaire and to provide a urine sample. SPE-HPLC-ESI-MS were used to determine the concentration of phthalates metabolites.We used ordinal Logistic regression of Windows SPSS16.0to analyzed data.Results(1) Optimizing the analyzing conditions. Optimizing the analyzing conditions including the samples pretreatment and HPLC method. Five phthalate monoesters are extracted from the urine sample using solid phase extraction method on the ELUT-Nexus cartridge under the optimum condition. Then flowed the elution solution to dry by nitrogen, and diluted them to200u L with pure water. A high performance-liquid-chromatography-tandem mass spectrometry (HPLC-MS) method was developed for the analysis three phthalate monoesters. The limit of detection (LOD) were:MMP:1.98ng/ml, MEP:0.98ng/ml, MBP:0.534ng/ml, MBzP:0.141ng/ml, MEHP:0.127ng/ml.(2) Determine the concentration of phthalates metabolites in the urine samples. Data shows that the detectable ratios of the5phthalate monoesters were:MMP and MEHP were the most prevalent (98.94%), followed by MEP (94.7%) and MBP (79.42%), MBzP were found in about65.26%of subjects. The median level and P25-P75level of MMP, MEP, MBP, MBzP, and MEHP were:44.74(24.16-80.27),11.68(4.33-27.86),10.13(1.83-22.93),0.48(LOD-1.78),37.85(17.90-70.89) ng/ml.(3) Analyzing the factors making pregnant women exposure to PAEs. Age was the possible influencing factors of DMP(OR=0.55,95%CI:0.32-0.94) and BBP (OR=4.01,95%CI:1.44-11.16; OR=5.08,95%CI:1.84-14.04), educational level was the possible influencing factors of DEP (OR=4.24,95%CI:1.56～11.53; OR=2.95,95%CI:1.04～8.40) and DBP (OR=0.3495%CI:0.12-0.98), smoking was the possible influencing factors of DEP (OR=1.86,95%CI:1.08～3.20) and DBP (OR=1.85,95%CI:1.06～3.21), drinking was the possible influencing factors of DMP(OR=3.85,95%CI:1.32～11.23), often using plastic containers in cooking or storage was the possible influencing factors of DEP (OR=1.69,95%CI:1.08～3.20), water was the possible influencing factors of DMP (OR=1.76,95%CI:1.04～2.97)ConclusionThis study has successfully optimized SPE-HPLC-ESI-MS to determine the level of PAEs. From the result of this study, PAEs has been detected in most pregnant women urine samples. This research has analyzed possible influencing factors on pregnant exposure to PAEs in pregnancy. Age, educational level, smoking, drinking water, often using plastic containers in cooking or storage was the possible influencing factors.