The Expression Of PTEN、PI3K And Ki-67in Endometrial Polyps And Associated Research

Objective Endometrial polyps is localized to the endometrial glands and stroma of benign hyperplasia, shown as a pedicled or sessile vegetation process to the intrauterine growth. Endometrial polyps can be classified as hyperplastic polyps, functional polyps and atrophic senile polyps, occasionally for the development of malignant tumor. This study based on different types of endometrial polyps and endometrial tissue specimens of PTEN tumor suppressor gene, PI3K kinase and Ki-67nuclear antigen immunohistochemical expression were detected, aims to further explore the possible pathogenesis of endometrial polyps.Methods Selection of Tianjin City Department of pathology from January to2010in Jinghai Hospital in2007December hysterectomy specimens and hysteroscopy on the endometrial polyps and their corresponding endometrial tissue specimens from a total of77cases. One of the normal proliferative phase endometrium of10cases, secretory phase endometrium in10cases, normal senile endometrium in10cases,25cases of hyperplastic polyps, functional polyps in10cases, atrophic senile polyps in12cases. Immunohistochemistry using two step method for detection of PTEN, PI3K and Ki-67in different types of endometrial polyps and endometrial tissue in the expression and distribution of. By using the SPSS13.0statistical software packages for data processing, P<0.05for the difference was statistically significant.Results1、PTEN expression in endometrial glandular epithelial cell cytoplasm, interstitial almost no expression. PTEN in the proliferative phase endometrium, hyperplastic polyposis of the positive expression rates were83.2%,50.1%, two groups compare, decreased, the difference was statistically significant (P<0.05). PTEN in secretory endometrium, functional polyps in the positive expression rates were87.1%,60.4%, two groups compare, decreased, the difference was statistically significant (P<0.05). PTEN in the senile endometrium, older polyp in the positive expression rates were67.4%,30.4%, two groups compare, decreased, the difference was statistically significant (P<0.05).2、PI3K is mainly expressed in endometrial glandular epithelial cell cytoplasm, interstitial almost no expression. PI3K in the proliferative phase endometrium. hyperplastic polyposis of the positive expression rates were54.9%,86.7%, two group, was increased, the difference was statistically significant (P<0.05). PI3K in secretory endometrium, functional polyps in the positive expression rates were54.1%,76.8%, two group, was increased, the difference was statistically significant (P<0.05). PI3K in the senile endometrium, older polyp in the positive expression rates were39.9%,60.9%, two group, was increased, the difference was statistically significant (P<0.05).3、Ki-67is mainly expressed in endometrial glandular epithelial cell nuclei. Ki-67in the proliferative phase endometrium, hyperplastic polyposis of the positive expression rates were25%,46.9%, two group, was increased, the difference was statistically significant (P<0.05). Ki-67in secretory endometrium, functional polyps in the positive expression rates were8.3%,5.2%, two groups, the difference was not statistically significant (P>0.05). Ki-67in the senile endometrium, older polyp in the positive expression rates were5.3%,4.8%, two groups, the difference was not statistically significant (P>0.05).4、Endometrial polyps in PTEN and PI3K was negatively correlated, correlation coefficient rs=0.525(P<0.05).Conclusion1. PTEN protein in endometrial polyps in cell plasma expression rate decreased, suggestive of endometrial polyps and the development of PTEN deletions may have some relationship.2. PI3K in endometrial polyps in cell plasma expression was increased significantly, tips in endometrial polyps during the evolution of PI3K, through its against apoptosis and promote proliferation of endometrial polyps may promote the formation and development of.3. Ki-67is a reflection of cell proliferation index. In proliferative endometrium polyp tissue Ki-67expression rate increased, prompting in a hyperplastic endometrial polyps, endometrial polyp formation may be associated with cell division and proliferation of.4. In endometrial polyps, the expression of PTEN and PI3K expression there is a negative correlation between them, the results may be the decrease of PTEN expression, the PI3K inhibition attenuates, thereby allowing the endometrial hyperplasia evolved into endometrial polyps.