Expression Of PTEN And PI3Kprotein In Endometrial Carcinoma And Its Chinical Significance By Tissue Microarray Technique

Objective:Application of tissue microarray technique PTEN and PI3K protein expression and clinicopathological features of endometrial cancer, the relationship between its expression correlation.Methods:Collection of specimens of 31 cases of endometrial adenocarcinoma as experimental group, endometrial hyperplasia specimens of 20 patients, cases of 11 normal endometrium specimens of the control group. All specimens were confirmed by pathology. Those were not been carried out preoperative radiotherapy, chemotherapy and hormone treatment. By using tissue microarray and immunohistochemistry to detect PTEN and PI3K protein in normal endometrium, endometrial hyperplasia and endometrial cancer tissues and to analyze PTEN, PI3K expression with various clinicopathological parameters of the relationship ,and the relationship between the PTEN and PI3K. Application SPSS10.0 statistical package for statistical analysis.Results:(1) In normal endometrium, the positive expression rates of PTEN and PI3K were 72.7% and 22.2%; In endometrial hyperplasia, the positive expression rate were 30% and 35%;In endometrial cancer, the positive expression rates were 22.6% and 80.6%. The rate of positive expression of PTEN and PI3K in Endometrial cancer and atypical hyperplasia compared with the rate in normal endometrium have significant differences (P﹤0.05).(2) The positive expression rates of PTEN in high-differentiated group and poorly differentiated group in endometrial cancer were 38.9% and 0%, there was significant difference between the two groups (P<0.05); The positive expression rates of PI3K in high-differentiated group and the poorly differentiated group in endometrial cancer were 61.1% and 100.0%. There was significant difference between the two groups (P< 0.05).(3) The positive expression rates of PTEN in the absence of myometrial invasion or invasion≤1 / 2 group and the depth of invasion>1 / 2 in the group were 41.2% and 0%, there was significant difference between the two groups (P<0.05); The positive expression rates of PI3K in the absence of myometrial invasion or invasion≤1 / 2 group and the depth of invasion>1 / 2 in the group were 58.8% and 100.0%, there was significant difference between the two groups (P< 0.05).(4) The the positive expression rates of PTEN in the absence of lymph node metastasis of endometrial carcinoma and metastasis were 30.0% and 9.1%, there was no significant difference (P >0.05); The the positive expression rates of PI3K in the absence of lymph node metastasis of endometrial carcinoma and metastasis were 65.0% and 100.00%, there was significant difference between the two groups (P <0.05).(5) Comparing with the rate of positive expression of PTEN and PI3K,it has shown a negative correlation (γ=- 0.631, P <0.05).Conclusion:1.One of the key factors in endometrial epithelial cells in the normal regulation of differentiation is the normal expression of PTEN. The lossing of PTEN may contribute to the development of endometrial cancer.2.High expression of PI3K was to promote the occurrence and development of endometrial cancer.3. The histological grade and tumor invasion of PTEN expression in endometrial cancer showed negative correlation. The lower of expression in PTEN, the higher in the degree of malignant tumors.4. The histological grade, tumor invasion and lymph node metastasis of PI3K expression in endometrial cancer showed positive correlation. The higher of expression in PI3K, the higher in the degree of malignant tumors.5.PTEN and PI3K expression was negatively related to the occurrence and development of endometrial cancer in antagonism.6.Tissue microarray for the Comparative Study of Multi-factor have advantages about high efficiency and good quality control.