Isolation And Identification Endophytic Fungi From Humulus Scandens And The Preliminary Studies On Antibacterial Activity Of Huniulus

Humulus Humulus scandens (Lour.) Merr and Ming Lala seedlings, as Sankohplants. The whole plant can be used as medicine, the dried whole plant clearing anddetoxifying, elimination bruises, antibacterial anti-inflammatory and other effects.Many scholars endophytes from medicinal plant extracts in many structures one afteranother novel compound, and most of them have unique biological activity, the drugendophyte research has become a hot spot. Based on this, the study of endophyticfungi Humulus conducted a preliminary study, the main results are as follows:1Isolated by homogenization and tissue slab method, using potato dextrosemedium, from Humulus roots, stems, leaves, petioles, and other organizations,21strains were isolated and purified from endophytic fungi. Measurement ofantibacterial activity by confrontation, that: one of the pathogens Staphylococcusaureus and Escherichia coli strains with antagonistic activity have two, named for theSL8and SS3.2Identification of endophytic fungi on Humulus colony, mycelium and sporesmorphological identification of fungal ribosomal RNA ITS sequence analysis of theidentification of molecular biology to determine the SL8is imperfect fungi sporessubphylum cluster stems Fusarium head Mortierella fungi. SS3was identified asTrichoderma, unknown species.3Optimized to enhance the antimicrobial activity for the purpose of thefermentation medium composition on the SL8to optimize test found that: the originalmedium of potato dextrose medium, medium volume80mL, Humulus Juice6%addition level, inoculum4%, the fermentation time of8d as a more suitable cultureconditions for this condition, SL8highest antibacterial activity on Staphylococcusaureus inhibition zone diameter of26mm.4The characteristics of the active substance Humulus broth of endophytic fungiin40~90℃range of strains of Staphylococcus aureus has a good inhibitory; and inpH4~8has a good antibacterial effect.5Separation of active substances SL8Initially confirmed by the chemicalcomposition of the active substance detection, the SL8fermentation broth activesubstances for the polysaccharides and within the lipids.Step by step extraction analysis of antibacterial substances the SL8antibacterial substances are mainlyconcentrated in the ethyl acetate extraction phase. Confirmed by IR spectroscopycontains active substances such as carbonyl and hydroxyl groups. Preliminarydetermination of active substances as polysaccharides.The experiment, there are a lot of Humulus endophytes, the study from21strainsof endophytic fungi were screened two high resistant strains belonging to Fusariumand Trichoderma, in the medium volume was80mL, inoculum4%6d fermentationtime for the culture conditions more suitable for this condition, SL8highestantibacterial activity, antimicrobial materials have good heat resistance and lightcharacteristics of acid, to provide a basis for further research information.