Magnetic Polymer Microsphere Technology And Its Evaluation In Removing Endotoxin

Several kinds of magnetic polymer microspheres were prepared in this articlebeacause the convenience brought by the magnetic polymer microsphere technologyin the separation. And their applications in adsorbing endotoxin were investigated.Themain results are as follows:Magnetic chitosan microsphere (MCM) was prepared through inverse suspensioncrosslinking and the preparation conditions were optimized. Endotoxin adsorptionexperiments were taken after grafted with histidine(His) and tetraethylenepentamine(TEPA). The result showed that the adsorption process of MCM-His fit the Langmuirmodel and the theoretical maximum adsorption capacity was2210.2EU/g. In thebovine serum albumin (BSA) solution, the endotoxin adsorption efficiency of thesetwo adsorbents(MCM-His and MCM-TEPA) was74.0%and58.2%, and the BSAadsorption efficiency was1.2%and3.7%, respectively.The magnetic chitosan anion exchange agent (ARMCM) was prepared withformaldehyde, glutaraldehyde as the crosslinker, and the preparation process wasimproved. The amino groups on the chitosan reserved after crosslinking can be usedas the functional group in adsorbing endotoxin. The results indicated that adsorptionbehavior fit the Langmuir model, R2=0.9996, and the maximum adsorption capacitywas1792.1EU/g. The endotoxin adsorption efficiency in the protein solution wasclose to80%and what’s more, the protein adsorption efficiency was only23.2%and2.0%respectively for acidic and basic proteins.At last, four magnetic endotoxin affinity adsorbents were prepared by dextranand agarose. The magnetic agarose microsphere coupling with TEPA(MAM-TEPA) had the largest adsorption capacity with1467.2EU/g.The adsorption results in theprotein solution indicated that these affinity adsorbents have certain selectivity inadsorbing endotoxin, and the endotoxin and protein adsorption efficiency ofMAM-TEPA was77.5%and17.3%, respectively.It is expected that these magnetic polymer microspheres will have a feasible anduseful application in adsorbing endotoxin from biological products.