Imunotoxicity Studies Of Nano-materials (MSNs And MWCNTs)Administrated By Local Injection To Mice

Background:Nanotechnology is increasingly widespread in the medical field, nanomaterials are also highly optimistic about the prospect of diagnosis and treatment of pancreatic cancer, however, immunotoxicity of nanomaterials has hindered its development. This experiment select the two nanomaterials which are closely related to application for treatment of pancreatic cancer as study object, mesoporous silica nanoparticles(MSNs) and multi-walled carbon nanotubes(WMCNTs), to explore the immune toxicity after local administration in the mice foot pad.Methods:Choose BALB/C female mice as experimental animal, all the mice were randomly divided into three groups, including high-dose group, low dose and control groups, MSNs and WMCNTs were local administrated in mice foot pad, then the immune function related indicators of mice were detected respectively in the14th days and60th days.(1) Observed the general situation, including death, irritability and lethargy.(2) Detected the changes of weight of the mice, organ mass of major organs, and the weight of lymph nodes.(3) Detected the liver and kidney function in mice by biochemical assay.(4) Detected the mice serum immunoglobulin, complement C3and inflammatory cytokines by Elisa assay.(5) Examined histopathologically the major organ damage.(6) Analyzed the splenic T cell subsets.(7) Detected T cell and B-cell proliferative capacity by MTT method.Results:(1) During the period of experiment, no mice died, and no ulcer, erosion or necrosis was observed on the MSNs and WMCNTs administration site.(2) Local application of MSNs and WMCNTs retarded the weight gain of mice, but did not affect the weight of organs and the draining lymph nodes.(3) Local application of MSNs and WMCNTs did not cause blood, liver and kidney dysfunction in the observation period.(4) Local application of MSNs and WMCNTs unchanged IgG, IgM, and IgA within the observation time. Complement C3and inflammatory cytokines decreased to varying degrees at60days compared to14days.(5) All major organs found no significant pathological changes.(6) Local application of MSNs and WMCNTs doesn’t cause abnormal ratio of T cell subsets in the spleens of mice.(7) MSNs and WMCNTs doesn’t affect the proliferative capacity of T cells and B cells. Conclusion:Local application of MSNs and MWCNTs, retard the growth of the body weight of mice, increase the body’s inflammatory cytokine levels temporarily, but no obvious primary immune toxicity.