The Expression Of Gr And Its Correlation With Nf-κB In OLP

Objective:To study the expression of glucocorticoid receptor (GR), and to investigate its role and its correlation with nuclear factor-kappa B (NF-κB) in OLP, finally provide new method of OLP therapy and lay a good foundation for specific drugs to improve the effect of glucocorticoid for patients with OLP.Methods:1. There were12cases of oral mucosa damages of OLP, among which6cases were reticular-plaque OLP, and the other6cases were erosive type. Meanwhile,4cases from healthy mucosas were as control. Protein and mRNA expression of GRa and GRβ were detected by immunohistochemical staining and real time reverse transcription polymerase chain reaction (real time RT-PCR) respectively.2. mRNA expression of p65and p50, the subunits of NF-κB, were detected by real time RT-PCR. And the relationship of GR and NF-κB in OLP was analyzed.Results:1. GR protein was mainly expressed in keratinocytes of oral mucosas in OLP.2. The quantity of protein and mRNA of GRa were significantly decreased in OLP compared with control groups (P<0.05). Oppositely, the quantity of GRβ protein was significantly increased in OLP compared with healthy mucosas (P<0.05). While the quatity of GRP mRNA in OLP was a little more than that in control mucosas, there was no statiatical difference in the two groups (P>0.05). The ratio of GRa and GRβ was significantly decreased in OLP (P<0.05).3. There were no statiscal differences in the quatity of GRa and GRβ mRNA between reticular-plaque OLP and erosive OLP. Nor was there in the ratio of GRa and GRβ (P>0.05).4. The quatity of p65and p50mRNA were significantly increased in OLP compared with healthy controls (P<0.05), but were no statistical differences between two types of OLP (P>0.05).5. There were negative correlations between GRa and p65or p50in OLP with statistical differences (P<0.05).Conclusions:1. GR protein is mainly expressed in keratinocytes of oral mucosas in OLP, suggesting GR may involve in the regulation of local pathological mechanism in OLP and keratinocytes could be the target cells for treatment with GR.2. The low expression of GRa in OLP interferes with the antiinflammatory mechanism of GC in local mucosas and impairs local anti-inflammatory effect, which probably lead to the reoccurrence of chronic inflammation in OLP.3. The low expression of GRa and the high expression of GRβ protein, together with low ratio of GRa and GRβ, suggest there maybe some correlation between glucocorticoid resistence and OLP.4. There is no significantly difference in the expression of GR subunits between two type of OLP, which infers there is no correlation between the condition of OLP and glucocorticoid resistence.5. The high expression of p65and p50in local damage of OLP implies NF-κB prossibly play a role in OLP by taking part in the chronic inflammation.6. The negative correlations between GRa and p65or p50in OLP provide basis for specific drugs targeted to both GR and NF-κB in OLP treatment.