ERIC-PCR Fingerprinting Of Structural Features Of Intestinal Flora In Ulcerative Colitis

ObjectiveTo establish DNA fingerprint of intestinal flora in ulcerative colitis and healthy subjects,and analyze the structural characteristics of such fingerprint.To explor the relationshipbetween the change of ulcerative colitis intestinal flora and occurrence and development ofdisease. To provide the basis for clinical treatment of UC.MethodsCollecting fifty-five cases UC patients individed into two groups, UC active and UCremission. and fifteen healthy people as control. The total DNA was extracted from thefecal samples, and Enterobacterial Repetitive Intergenic Consensus sequence polymerasechain reaction(ERIC-PCR) was used to set up the DNA fingerprint of intestinal flora. UseUVP gel electrophoresis analysis software statistical analysis the UC active and UCremission and normal controls were intestinal diversity and similaty.Using the SPSS16.0software and one-way ANOVA to process the data.ResultsFrist, the intestinal bacteria DNA fingerprint electrophoresis. The overall differenceexists among the UC active and UC remission and normal controls. UC remission andnormal controls have more ERIC-PCR DNA fingerprints than UC active, and the brandshave no uniform trend. The UC active has less brands, and the main brands of thetwenty-six samples appear at the place of zero point seven.Second, the statistical resultsdealed with the UVP gel electrophoresis analysis software. Compared to other groups, theUC active had significant difference. And UC remission and normal controls’ intestinalflora intestinal structure diversity no significant difference.ConclusionUC active group’s diversity of intestinal flora had a certain degree of difference fromother two groups.