Effect Of Tumor-associated Macrophages (TAMs) On The Growth And Invasion Of Osteosarcoma

ObjectiveTo observe the effect of tumor-associated macrophages (TAMs) on the growth and invasion of osteosarcoma, and to approach the mechanism of that, in order to provide according to clinical medication.Methods20specimens of osteosarcoma with complete follow-up data were collected from patients who underwent surgery at the third People’s Hospital and the medical college of Qingdao university ffiliated hospital from January2010to Sept2011.Immunohistochemistry was used to investigate the location and contents of TAMs expressing CD206in osteosarcoma tissue and normal tissue. The expression of MCP-1,VEGF and MMP-9was detected in tissue by immunohistochemistry, to observe the effect of TAMs on the tumor invasion and angiogenesis of osteosarcoma. The relationship of MCP-1,VEGF and MMP-9expression and CD206were analysised.ResultsTAMs distributed in the interstitium of osteosarcoma tissue, and diffused distribution in normal tissue. The expression of CD206in osteosarcoma tissue was significantly higher than that in normal tissue (P<0.01). The positive expression of MCP-1,VEGF and MMP-9expression were significantly higher in osteosarcoma tissue (P<0.01)..The positive expression of MCP-1,VEGF and MMP-9was closely associated with TAMs using the S-pearman rank correlation analysis(r=0.687,0.562,0.496,P<0.01).Conclusion1. MCP-1could be produced by both tumor cells and macrophage and he expression of CD206、MCP-1in osteosarcoma were highly. It suggested that MCP-1could attract more macrophage to tumor site and played an important role in accelerating tumor angiogenesis.2. The expression of VEGF in osteosarcoma was highly and there was significent positive correlation of TAMs. It suggested that TAMs could promote tumor angiogenesis and recurrence by up-regulating the expression of VEGF.3. The expression of MMP-9in osteosarcoma was highly and there was significent positive correlation of TAMs. It suggested that TAMs could promote metastasis by up-regulating the expression of MMP-9.4. The expression of CD206and MCP-1in osteosarcoma was highly and there was significent positive correlation of VEGF and MMP-9. This suggested that the expression of CD206and MCP-1should be used as a helpful index for metastasis and the tumor angiogenesis of osteosarcoma.