Clinical Significance Of Quantitative Detection Of Epstein-Barr Virus DNA In Children With EBV Infection

Objective:To investigate and analyze the clinical significance of diagnosing children Epstein Barr virus (EBV) infection in children with fever by Real-time quantitative PCR.Methods:Fluorescence quantitative polymerase chain reaction was used to detect EBV load in peripheral blood of205children with fever. Atypical lymphocyte in peripheral blood shaped lymphocytes, routine blood test, and liver and kidney function indicators were examined and the major clinical manifestations of EBV infection was combined for comprehensive analysis.Results:①The42out of205cases with fever were positive for EBV DNA in peripheral blood, the positive rate was20.5%, and23males and19females, the difference wasn’t significant (χ2=1.0246, P=0.3114). EBV DNA positive rate from1to3years and from4to6years old were higher, there was no significant differences among different ages (X2=1.5257, P>0.05).②205cases of children with fever, white blood cells, liver and renal function abnormality in EBV DNA-positive children is slightly higher than EBV DNA-negative children, but the difference was not statistically significant(P>0.05), cardiac enzymes is higher(χ2=1.0246, P=0.0348).③Compared with163cases of EBV DNA-negative children with fever, EBV DNA-positive children with fever atypical lymphocytes rate was significantly higher than that EBV DNA-negative children with fever, and the difference was statistically significant (χ=31.3551, P<0.0001).④The42cases of EBV DNA-positive children with fever starting main clinical manifestations such as angina, swollen lymph nodes and hepatosplenomegaly abnormality was significantly higher than that of EBV DNA-negative children, and the difference was significant (P<0.05).Conclusion:Real-time PCR detection of EBV DNA load contributes to the early diagnosis of EBV infection and can reduce the blindness of the clinic. It has certain guiding significance for clinical diagnosis and treatment. Atypical lymphocytes detection in children with fever as well as the first major clinical manifestations, such as angina, swollen lymph nodes, liver and spleen enlargement can be used for diagnosis of EBV infection. Laboratory blood count, liver function, kidney function, and cardiac enzymes checks can reflect the condition of the children, and can be used for monitoring treatment in children in the course of treatment.