Transcriptomics Study Of Chronic Hepatitis B Wity Liver Stagnation And Spleen Deficiency Syndrome And Spleen And Stomach Damp-heat Syndrome

Objective:apply the gene chip technology to research changes in the gene expression of the light or moderate Chronic Hepatitis B with liver stagnation and spleen deficiency syndrome, spleen-stomach Damp-heat syndrome(P<0.05), filter out the differential genes relating to syndrome type, find the different gene function in gene expression, and provide a basis for objective research and Differential Treatment of TCM syndromes of chronic hepatitis B.Methods:In this study, we choose the syndrome of liver stagnation and spleen deficiency and syndrome of spleen-stomach damp-heat,which are the common clinical syndrome of chronic hepatitis B for transcriptomics studies. On the basis of pre-group clinical research and literature findings.The object of study are the syndrome of liver stagnation and spleen deficiency, syndrome of spleen-stomach damp-heat and healthy people collected by Chengdu Infectious Disease Hospital, West China Hospital of Sichuan University and Affiliated Hospital of Chengdu University of Traditional Chinese Medicine.The patients with chronic hepatitis B were divided into group of liver stagnation and spleen deficiency syndrome, group of spleen-stomach damp-heat syndrome, at the same time a normal crowd-control group was also set. Add every patient’s6ml peripheral blood into the50ml centrifuge tube coated EDTA, join the red blood cell lysis liquid, centrifugation, washing, join TRIZOL, transfer the isolated white blood cells into1.5mlEP tube, get the studied samples, and then put the samples in-80℃refrigerator to save after numbered. The samples are buried in a foam box with dry ice after packaged timely,then strictly sealed, sent to Shanghai Bohao biological technology co., Ltd via express air. Technicians of Shanghai bohao biotechnology co., Ltd finish quality testing of the samples, all qualified,then come into the experimental stage, extract RNA, RT (Reverse transcription) to cDNA, complete preparation of gene chip by using gene chip hybridization and scanning. Finally, in the SAS software, complete screening of different genes of liver stagnation and spleen deficiency syndrome and spleen-stomach Damp-heat syndrome, and analyze the significant differences in gene function of differential genes.Results:(1) According to the standard P-value <0.05, we screened1401differentially expressed probe related to liver depression and spleen deficiency syndrome,592up-regulated and809down among them. There were939differentially expressed genes with functional annotation.(2) According to the P-value <0.05standard, filtered out2011differential expression probe related to liver stagnation and spleen deficiency syndrome, including807up-regulated and1204down,1376differentially expressed genes with functional annotation.(3) Significant analysis to the differential gene function liver stagnation and spleen deficiency syndrome-related found that:the main function differential gene expressing are response to external stimulus, carbohydrate binding, multicellular organismal response to stress, cell development, growth and development and so on.(4) Significant analysis to the differential gene function associated with spleen-stomach damp-heat syndrome showed that:the main function differential gene expressing are anatomical structure formation involved in morphogenesis, regulation of locomotion, cell motion, cell adhesion, regulation of localization, regulation of homeostatic process.Conclusion:liver stagnation and spleen deficiency syndrome and spleen-stomach Damp-heat syndrome of chronic hepatitis B have different genes related to the syndrome type. Differential genes are involved in expression of many gene function, and gene function differential genes express have differences with each other in the two syndrome types.This have the referential value to the objective research on traditional Chinese medicine syndrome.