| BACKGROUND:Bone marrow mesenchymal stem cells(BMMSCs) has the potentiality of multiple differentiation. Our former research results showed that BMMSCs can be induced to vascular smooth muscle-like cells(VSMLCs) and vascular endothelium-like cells(VELCs) and the cells are compatible with PGA scaffolds.Objective:To investigate the possibility of constructing small diameter artery by means of tissue engineering in vivo. Methods:The mixture of seed cells and scaffold were produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid (PGA) collagen scaffold. The two layers were separated by ECMgel. The tissue engineering blood vessels were examined by both gross and histology with HE staining and immunofluorescence.Results:Histological analysis of the tissue engineering blood vessel wall revealed a typical artery structure, including endothelium, media and adventitia. Eight weeks after implantation, the labeled seed cells by Brdu were found in the three layers of the vessel wall. Conclusion:The subcutaneous tissue was a good bioreactor to construct tissue engineering blood vessels. Histology of tissue engineering blood vessels was similar to natural vessel. |
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