Stuty On The Inhibition Of Erythropoietin To Inflammation Of Focal Cerebral Ischemia-Reperfusion Injury In Rats

ObjectiveTo investigate the expression of NF-κB, VCAM-1and ICAM-1in thehippocampal CA1area of rats with focal cerebral ischemia-reperfusion and theinfluence of EPO on the expression of NF-κB, VCAM-1and ICAM-1; To explorethe inhibition of EPO to inflammation with focal cerebral ischemia-reperfusioninjury in rats.Methods70SD rats were randomly divided into5groups (n=14): SH, IR, PTDC,EPO, PDTC+EPO group. Rats were made into the modal of focal cerebralischemia-reperfusion by occluding the middle cerebral artery. The IR group,PDTC group, EPO group and PDTC+EPO group were reperfused for24h afteroccluding the middle cerebral artery for2hours. The rats accepted EPO(3000U/kg) and PDTC (100mg/Kg) after occluding the blood flow for2h; While,the rats of the SH group and IR group accepted the equal normal saline.Observe the scores of neurological impairment of the rats after occluding theblood flow for2h and reperfused for24h. The brain tissues of rats wereobserved by HE staining, immunohistochemical staining and Western Blot.ResultsThe scores of neurological impairment: The nervous function of rats in SHgroup was normal. Contrast to the SH group, the scores of neurologicalimpairment of rats in IR group were increased (P<0.01). Contrast to the IR group, the scores in EPO group, PDTC group and PDTC+EPO group weredecreased (P<0.01). Contrast to the PDTC group, the scores in EPO group andPDTC+EPO group were decreased (P<0.01). There was no statisticalsignificance between EPO and PDTC+EPO group (P>0.05).HE staining: The neurons in the hippocampal CA1area of rats in SH groupaligned closely and orderly, the morphology normal; the cytoplasm was silver;the nuclear was relatively round, the staining clearly; the nucleolus was obvious.In the IR group, the neurons aligned disorder and the morphology was irregular;the cytoplasm wasn’t bright, varying degrees of degeneration pyknosis necrosis.In the PDTC group, EPO group and PDTC+EPO group, the neuronsmorphology were ameliorated, and EPO group and PDTC+EPO group weresignificant than other.Immunohistochemical staining: In the SH group, the expression of NF-κB innuclear was little, and the expression of VCAM-1and ICAM-1at vascularendothelial cell was also little. In the IR group, the expression of NF-κB,VCAM-1and ICAM-1was increased obviously in comparison to the SH group(P<0.01). Contrast to the IR group, the expression of NF-κB, VCAM-1andICAM-1in the PDTC group, EPO group and PDTC+EPO group was decreasedobviously (P<0.01). The expression of VCAM-1and ICAM-1in PDTC group washigher than SH group (P<0.01). The expression of VCAM-1and ICAM-1in EPOgroup and PDTC+EPO group was lower than PDTC group (P<0.01). There wasno statistical significance between EPO and PDTC+EPO group (P>0.05).Western blot: In the SH group, the expression of NF-κB, VCAM-1andICAM-1was little. In the IR group, the expression of NF-κB, VCAM-1andICAM-1was increased obviously in comparison to the SH group (P<0.01).Contrast to the IR group, the expression of NF-κB, VCAM-1and ICAM-1in thePDTC group, EPO group and PDTC+EPO group was decreased obviously(P<0.01). The expression of VCAM-1and ICAM-1in PDTC group was higher than SH group (P<0.01). The expression of VCAM-1and ICAM-1in EPO groupand PDTC+EPO group was lower than PDTC group (P<0.01). There was nostatistical significance between EPO and PDTC+EPO group (P>0.05).ConclusionsICAM-1and VCAM-1play an important role in the inflammation with focalcerebral ischemia-reperfusion injury in rats. The induction and expression ofICAM-1and VCAM-1could be accomplished through the pathways ofnon-NF-Κb except for NF-κB in the inflammation. EPO could inhibit the twopathways in order to play a neuroprotective action.