Investigation On RCG On Angiogenesis In Mouse With Malignant Melanoma

ObjectiveTo investigated effect of RCG on the mice formation of MalignantMelanoma and to explore its mechanism.MethodsB16melanoma cells were cultivated in vitro, then inoculated in right oxterin the C57BL/6mice. The model of mouse with Malignant Melanoma wasmade. The mice were randomly divided into five groups, included normal salinegroup, RCG group at varied concentrations and CTX group. Each groupconsisted of eight mice.High dosage group(700mg/kg), middle dosagegroup(300mg/kg), low dosage groups of RCG (100mg/kg) and CTX (60mg/kg)group were injected with RCG and CTX for21days. after the tumor model wasmade. The RCT group once a day, the CTX group once a week. The grouth oftumor was observed and the inhibitory rate of tumor was measured. The proteinexpression of VEGF, MMP-9and VE-cadherin in tumor were determined withwestern blot, the gene expression of VEGF and MMP-9were detected withRT-PCR.The protein expression of VE-cadherin was measured immunohistoch-emical.ResultsThere were significant differences from inhibitory rates of primary tumor Between RCG group and Saline group(P〈0.01). The protein expression level ofVEGF, MMP-9and VE-cadherin in RCG groups were lower significantly thansaline group(P〈0.01), the gene expression level of VEGF and MMP-9in RCGgroups were lower significantly than saline group(P〈0.01).ConclusionRCG could effectively inhibit the growth of Malignant Melanoma in C57B-L/6mouse, which was probably the result that RCG reduced the proteinexpression and gene expression of VEGF, MMP-9and VE-cadherin. Meanwh-ile, the effects of RCG were dose-dependent to some degree, and theimprovement of the high doses group was the most ideal.