Effects Of Intervention Of Shou Wu Yan Shou Dan In Osteoporosis Of The Ovariectomized Rat On Experimental Study

ObjectiveObserving bone mineral density, serum calcium, phosphorus, serumalkaline phosphatase, urinary pyridinoline, urinary creatinine, urinarypyridinoline/urine creatinine ratio, osteocalcin, type Ⅰ procollagen amino-theend of the propeptide, type Ⅰ collagen cross-linked carboxy-terminaltelopeptide and bone morphology at Shou Wu Yan Shou Dan onovariectomized rat, and its effects of intervention on osteoporosis in rats aftercastration.Methods60female SD rats in3-month-old were randomly divided into six groups,sham operation group, model group, estrogen group, Shouwu Yanshou Danhigh, medium and low-dose group. In addition to the sham operation group, Allwas to be ovariectomized at5th days after administration, Urine was taken tomeasure urinary pyridinoline (PYD) and urinary creatinine (U-Cr) at6th weeks;serum was taken to measure calcium phosphorus, alkaline phosphatase,osteocalcin (BGP), type Ⅰ procollagen amino-terminal peptide (PINP), type Ⅰcollagen cross-linked carboxy-terminal telopeptide (ICTP), Bone mineraldensity of femoral was taken to measure at12th weeks. Femur was fixed with4%paraformaldehyde solution in24h, and was decalcified with10%EDTA，and was embedded in paraffin，and was made of tissue sections on5mm thick,and was stained with HE, and then was taken to view pathologicalmorphology.Results1, Serum biochemical indices and bone mineral density Difference ofall groups of calcium, phosphorus, serum alkaline phosphatase wasstatistically insignificant (P>0.05), model group: type Ⅰ procollagenamino-terminal propeptide and osteocalcin increased(P <0.01), type Ⅰ collagencross-linked carboxy-terminal peptide was significantly increased (P <0.01),ratio of urinary pyridinoline/urine creatinine increased (P <0.01), bone mineraldensity was reduced (P<0.01); The groups of Chinese medicine.and thefemale hormone group, type Ⅰ procollagen amino-terminal telopeptide andosteocalcin was significantly lower (P <0.01), Osteocalcin of high dose groupof Chinese medicine is lower than the other groups in traditional Chinesemedicine (P<0.01). And type Ⅰ collagen amino-terminal propeptide of high dosegroup of Chinese medicine was lower than low dose group of Chinesemedicine (P <0.05); type Ⅰ collagen cross-linked carboxy-terminal peptidewas significantly lower (P <0.01), type Ⅰ collagencross-linked carboxy-terminalpeptide of high dose group of Chinese medicine was significantly lower thanthe other groups of traditional Chinese medicine (P <0.01), the ratio of urinarypyridinoline/urine creatinine was significantly lower (P <0.01),,the ratio ofurinary pyridinoline/urine creatinine of high dose group of traditional Chinesemedicine was lower than the other groups of traditional Chinese medicine (P<0.01), bone mineral density was increased (P <0.01), bone mineral density ofhigh dose group of traditional Chinese medicine was greater than other groupsof traditional Chinese medicine (P <0.01). Efficacy of The Shou Wu Yan ShouDan show clear dose-dependent.2, The pathological changes: sham operation group is no significanthistological changes; the model group: trabecular bone is thin, the integrity ofbone lamellar structure is loose and disorganized. the bone is less vertical lines. Chinese medicine group and the estrogen group: trabecular bone ismore sturdy, the integrity of bone lamellar structure is dense, and arranged inan orderly manner, the bone is more vertical lines.ConclusionsAfter the castrated rats were made by Shou Wu Yan Shou Dan, type Ⅰprocollagen amino-terminal telopeptide and osteocalcin were significantlyreduced; type Ⅰ collagen cross-linked carboxy-terminal peptide wassignificantly reduced; the ratio of urine pyridinoline/urine creatinine wassignificantly lower; bone mineral density was increased. The pathomorphologywas improved, Shouwu Yanshou Dan have effect of intervention atosteoporosis after ovariectomized rat...