| With today’s advanced technology, ionizing radiation can be found almost everywhere in human life. Severe diseases can be caused by inevitable damages from radiations such as chemotherapy operated on patients with malignant tumor, radiative working environment, and space radiation on the astronauts. How to reduce radiation damage and proctet human body away from radiation has become an important subject to the medical researchers and other medical staff of prevention programs. It has been a new hot topic for the medical researches on radiation protection to find out a safe, effective, stable, low-toxic, and affordable natural radioprotective agents. It has caught the eye of the related researchers to develop low-toxic, effective, and practical radiation preventor from China’s abundant medical herb resources. This is a new orientation and trend for the study and development of new medicines.Objective:To investigate the effects of Pueraria lobata flower Polysaccharide (PLFP) on X-irradiated mice and research the anti-radiation effects of PLFP on irradiation-demaged mice.Methods:Establish X-irradiation damage models of mice and observe the anti-irradiation effects of PLFP.60healthy male mice are divided randomly into six groups (Normal control group, low-dose PLFP group, high-dose PLFP group, X-irradiated control group, low-dose PLFP X-irradiated group, and high-dose PLFP X-irradiated group). For the mice of low-dose PLFP group, high-dose PLFP group, PLFP are continuously fed for14days. For the normal control groups and X-irradiated control group, the mice are fed with distilled water. The mice were irradiated with5.0Gy X Ray after the last feeding with PLFP.24hours later of radiation, the mice are to be killed, and Peripheral blood leukocytes, spleen index, and Thymus index are going to be measured; The formation of chromosomes micronuclei of the mice is to be measured by Giemsa staining method and the quantity of the bone marrow DNA is to be measured by spectrophotometry; the SOD activity in the liver tissue of mice is to be measured with Xanthine oxidase method,and the GSH-Px activity is to be measured with Dithio-bis-nitrobenzoic acid colorimetric method;the spectrophotometry of molybdate is to be used to detect the CAT activity and the MDA content is to be detected with the method of TBA colorimetric.Results:Comparing with the irradiated control group, the damage on bone marrow micronucleus rate has been notably reduced by the PLFP and the radiation damage on bone marrow DNA can be reduced too; the activity of SOD, GSH-px and CAT in the liver of high-dose PLFP irradiated mice was significantly increased, and the MDA level was apparently lowered. It is showed that the PLFP can not only reduce the X-ray damage to the Hematopoietic system, but also increase the antioxidant capacity of irradiated mice.Conclusion:This paper studies the effects of PLFP on the hematopoietic system and the antioxidative capacity of X-irradiated mice. It proves that PLFP can reduce the radiation damage, whose mechanism of action may be relevant to enhancing the oxidative resistance and DNA protection. Conclusions from the research are as follow:1. Both high dose and low dose of PLFP have notable effects on reducing the bone marrow micronucleus rate of irradiated mice and the demage of chromosome caused by irradiation.2. The DNA damage of irradiated mice can be reduced by high dose of PLFP.3. The activity of SOD and GSH-Px of irradiated mice can be increased by high dose of PLFP.4. The CAT activity of irradiated mice can be increased by high dose of PLFP.5. The MDA content of irradiated mice can be decreased significantly by high dose of PLFP. |
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