| BACKGROUNDThe drug pair interaction method is widely adopted in western medicine interaction assessment method, include:The classical isobologram approach; Tallarida, R.J; The median-effect method; The universal response surface analysis; ANOVA; Q value method, et al. For the evaluation of drug interaction methods in few studies and few in traditional Chinese medicine. Drug interactions method is a rigorous mathematical proof, has a wealth of application experience, a complete theoretical system, and able to give the entire dose range of drug interactions. For the compatibility of domestic research methods of factorial analysis, orthogonal analysis method, uniform design, Orthogonal t-value method, cluster analysis, logistic model to evaluate the method, et al. These methods have different characteristics, but the lack of complete scientific evidence and theoretical system, there is a disconnect and foreign.Ephedra decoction flavor is not respected famous dynasties in physician prescription, calendar version of the textbooks used to clarify the principle of prescription composition of the preferred prescription. Ephedra soup-based Clinical addition and subtraction changes are derived from a series of ephedra soups side. Xiao Qing Long Tang large XQLD. ephedra combined with intraoperative soup Mahuangfuzixixin Tang. Ma Xing Shi Gan Tang and so on. This paper begins with the drug to start, combined pharmacodynamic drug interactions, respectively, to explore the Herbal ephedrae-Radix aconiti lateralis praepareta and Herbal ephedrae-Cinnamomi ramulus this two drugs on the repair mechanism. Study traditional Chinese medicine mechanism of action in order to provide a more clear thinking, and international integration.OBJECTIVEProvide a clearer idea of the drug Herbal ephedrae-Radix aconiti lateralis praepareta and Herbal ephedrae-Cinnamomi ramulus pharmacodynamic interaction study of Chinese herbal medicines.METHODS1. DrugsHerbal ephedrae purchased from Guangzhou zhixin Pharmaceutical Co., Ltd. Origin:Jilin, batch number:20101002; Radix aconiti lateralis praepareta purchased from Sichuan jiangyou zhongba aconiti technology development, batch number:101002; Cinnamomi ramulus bought from guangdong province medicinal materials company, origin:guangxi, batch number:20101201; Aspirin, shu tai sheng biological pharmaceutical Co., LTD. God, batch number:110701:Metamizole Sodium Tablets guangdong south China pharmaceutical group Co., LTD., batch number:110115; Dry yeast. Angela yeast stock company.2. AnimalsKM mice, SPF level,18-22g;SD rats, SPF level,160-200g, male. All animals are provided by the Animal Center of Southern Medical University. Qualified SCXK number:guangdong2006-0015. 3. Outcome measures3.1Herbal ephedrae-Radix aconiti lateralis praepareta to pharmacodynamics experiment medicine3.1.1Herbal ephedrae-Radix aconiti lateralis praepareta to aconite different group urgent poison experiment3.1.2Herbal ephedrae-Radix aconiti lateralis praepareta to the Tightness of acetic acid in mice medicine body torsion to influence the reaction3.1.3Herbal ephedrae-Radix aconiti lateralis praepareta to the mice medicine radiant heat guy to influence the reaction3.1.4Herbal ephedrae-Radix aconiti lateralis praepareta interaction experiment proved3.2Herbal ephedrae-Cinnamomi ramulus to pharmacodynamics experiment medicine3.2.1Herbal ephedrae-Cinnamomi ramulus to aconite different group urgent poison experiment3.2.2Herbal ephedrae-Cinnamomi ramulus to the Tightness yeast hot rat antipyretic influence3.2.3Herbal ephedrae-Cinnamomi ramulus interaction experiment proved3.3The aconitine and compatibility of methamphetamine to the preliminary research on the toxicity of zebrafish heart4. Statistical MethodsStatistical software SPSS13.0was used to analyze the experimenial data.The experimental data were indicated by mean±standard deviation (±s). Repeated measures variance analysis and the single-factor analysis of variance(ANOVA) were used to analyze the data. LSD method was used in multiple comparisons between groups when variance is homogeneous. Dunnett’s T3method was used when variance is not homogeneous.Chi-square test was used to deal with count data.P<0.05indicates that the data have statistical significance.RESULTS1Herbal ephedrae-Radix aconiti lateralis praepareta to pharmacodynamics experiment medicine1.1Herbal ephedrae-Radix aconiti lateralis praepareta to aconite different group urgent poison experimentHerbal ephedrae-Radix aconiti lateralis praepareta1:0.5group of LD5o119.1479g/kg, Herbal ephedrae-Radix aconiti lateralis praepareta1:1group of LD50117.4859g/kg, Herbal ephedrae-Radix aconiti lateralis praepareta1:2group of LD50115.6288g/kg, Herbal ephedrae group of LD50136.3061g/kg, Radix aconiti lateralis praepareta group of LD50124.4897g/kg. The median-effect method is used Herbal ephedrae-Radix aconiti lateralis praepareta urgent poison experiment and using the software Calcusyn processing of data. Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group small dose antagonist, large doses of a total sum of the trend. Herbal ephedrae-Radix aconiti lateralis praepareta1:1group in small doses of a total sum of the trend, high-dose antagonist; Herbal ephedrae-Radix aconiti lateralis praepareta1:2group obvious lateral compatibility antagonist, In the same effect,the size of the CI order:Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group <Herbal ephedrae-Radix aconiti lateralis praepareta1:1group<Herbal ephedrae-Radix aconiti lateralis praepareta1:2group. Along with the increase of the content of Radix aconiti lateralis praepareta, Herbal ephedrae and compatibility of Radix aconiti lateralis praepareta acute toxicity of antagonist obviously increased.1.2Herbal ephedrae-Radix aconiti lateralis praepareta to the rightness of acetic acid in mice medicine body torsion to influence the reactionThe data of mouse writhing latency were analyzed by one-way ANOVA. It is shown that there was significant difference among groups(F=6.349, P=0.000) in mouse writhing latency, LSD method was used for further analysis; and there was significant difference among groups(F=4.438, P=0.001) in the data of mouse writhing, LSD method was used for further analysis. In the data of mouse writhing latency, compared with the model group, indomethacin. Radix aconiti lateralis praepareta2group,in the high-, and medium-dose Herbal ephedrae-Radix aconiti lateralis praepareta1:1groups, Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group, Herbal ephedrae-Radix aconiti lateralis praepareta1:2group and all the party group were significantly decreased(P<0.05). In the data of mouse writhing, compared with the model group, indomethacin, Herbal ephedrae group, Radix aconiti lateralis praepareta1group, Radix aconiti lateralis praepareta2group, in the high-, and medium-dose Herbal ephedrae-Radix aconiti lateralis praepareta1:1groups, Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group, Herbal ephedrae-Radix aconiti lateralis praepareta1:2group and all the party group were significantly decreased(P<0.05). Herbal ephedrae-Radix aconiti lateralis praepareta1:1groups, Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group,Herbal ephedrae-Radix aconiti lateralis praepareta1:2group and all the party group multiple comparison was no significant difference(P>0.05).1.3Herbal ephedrae-Radix aconiti lateralis praepareta to the mice medicine radiant heat guy to influence the reactionThe drift time of mice were analyzed by one-way ANOVA. It is shown that there was significant difference among groups(F=7.294,P=0.000) in mouse writhing latency. LSD method was used for further analysis.In the drift time of mice, compared with the model group, indomethacin. Radix aconiti lateralis praepareta0.5group. Radix aconiti lateralis praepareta1group. Radix aconiti lateralis praepareta2group, in the high-, and medium-dose Herbal ephedrae-Radix aconiti lateralis praepareta1:1 groups. Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group, Herbal ephedrae-Radix aconiti lateralis praepareta1:2group and all the party group were significantly decreased(P<0.05). Herbal ephedrae-Radix aconiti lateralis praepareta1:1groups. Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group Herbal ephedrae-Radix aconiti lateralis praepareta1:2group and and all the party group multiple comparison was no significant difference(P>0.05).1.4Herbal ephedrae-Radix aconiti lateralis praepareta interaction experiment proved The median-effect method is used Herbal ephedrae-Radix aconiti lateralis praepareta and using the software Calcusyn processing of data. According to experiment result front efficacy, using Herbal ephedrae-Radix aconiti lateralis praepareta1:1group of aconite correlation. Experiment with different doses of ephedra, Radix aconiti lateralis praepareta, and Herbal ephedrae-Radix aconiti lateralis praepareta.Obtained by the Calcusyn software processing, In the analgesic effect is less than80%,CI<1, Herbal ephedrae and Radix aconiti lateralis praepareta in analgesic effect on the compatibility of synergistic role.2Herbal ephedrae-Cinnamomi ramulus to pharmacodynamics experiment medicine2.1Herbal ephedrae-Cinnamomi ramulus to aconite different group urgent poison experimentHerbal ephedrae-Cinnamomi ramulus1:0.5group of LD50138.1220g/kg, Herbal ephedrae-Cinnamomi ramulus1:1group of LD50233.2471g/kg, Herbal ephedrae-Cinnamomi ramulus1:2group of LD50218.5646g/kg, Herbal ephedrae group of LD50139.3681g/kg, Cinnamomi ramulus group of LD50124.4897g/kg. The median-effect method is used Herbal ephedrae-Cinnamomi ramulus urgent poison experiment and using the software Calcusyn processing of data. Herbal ephedrae and Cinnamomi ramulus Three compatibility group all have antagonist role, In the same effect,the size of the CI order:Herbal ephedrae-Cinnamomi ramulus1:0.5group<Herbal ephedrae-Cinnamomi ramulus1:1group<Herbal ephedrae-Cinnamomi ramulus1:2group. Along with the increase of the content of Cinnamomi ramulus. Herbal ephedrae and Cinnamomi ramulus compatibility of acute toxicity antagonist obviously increased.2.2Herbal ephedrae-Cinnamomi ramulus to the Tightness yeast hot rat antipyretic influenceThe data of rats after administration of different time temperature increase were analyzed by two-way analysis of variance with repeated meseasures:there was interaction between the time point and the groups(F=5.973,P=0.000), main effects between groups (F=14.417, P=0.000) and main effects between the time groups (F=48.275, P=0.000). In rats after administration of two hours, compared with the model group, Analgin, Aspirin, in the high-, and medium-dose Herbal ephedrae-Cinnamomi ramulus1:1groups were significantly decreased(P<0.05). In rats after administration of three hours, all treatment groups compared with model group were significant differences (P<0.05). Herbal ephedrae-Cinnamomi ramulus1:0.5group. Herbal ephedrae-Cinnamomi ramulus1:1group, Herbal ephedrae-Cinnamomi ramulus1:2group and all the party group multiple comparison was no significant difference(P>0.05).2.3Herbal ephedrae-Cinnamomi ramulus interaction experiment provedThe median-effect method is used Herbal ephedrae-Cinnamomi ramulus and using the software Calcusyn processing of data.. According to experiment result front efficacy, using Herbal ephedrae-Cinnamomi ramulus1:1compatibility group continue to study Herbal ephedrae and Cinnamomi ramulusof compatibility relations, selected Herbal ephedrae, Cinnamomi ramulus and Herbal ephedrae-Cinnamomi ramulus compatibility than different doses of the experiment. CI value Calcusyn software processing to determine drug interactions, by CI<1, so the combination of Herbal ephedrae-Cinnamomi ramulus synergistic effect.3The aconitine and compatibility of methamphetamine to the preliminary research on the toxicity of zebrafish heartWith the increasing of the concentration of the aconitine, zebrafish embryonic cardiac toxic increase. The same concentration, with the aconitine role the extension of time, the heart of the embryo toxicity. Ephedrine and aconitine compatibility, along with the increasing of the concentration of methamphetamine, embryonic cardiac toxic increase.CONCLUSION1、Use Calcusyn software processing Herbal ephedrae and compatibility of Radix aconiti lateralis praepareta the acute toxicity of the experimental results, which proved the acute toxicity of compatibility with the main role for antagonist, In the same effect, compatibility of the strength of the antagonist group size:Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group<Herbal ephedrae-Radix aconiti lateralis praepareta1:1group<Herbal ephedrae-Radix aconiti lateralis praepareta1:2group. Along with the increase of the content of Radix aconiti lateralis praepareta. Herbal ephedrae and compatibility of Radix aconiti lateralis praepareta acute toxicity of antagonist obviously increased.2、Prove that Herbal ephedrae-Radix aconiti lateralis praepareta on the analgesic effect of acetic acid writhing test and the radiant heat tail flick test. Herbal ephedrae-Radix aconiti lateralis praepareta1:1groups. Herbal ephedrae-Radix aconiti lateralis praepareta1:0.5group, Herbal ephedrae-Radix aconiti lateralis praepareta1:2group and all the party group multiple comparison was no significant difference(P>0.05). 3、Judgment of drug relationship between CI values obtained by the Calcusyn software processing, in the analgesic effect is more than80%, CI<1, Herbal ephedrae and Radix aconiti lateralis praepareta in analgesic effect on the compatibility of synergistic role.4、Use Calcusyn software processing Herbal ephedrae and compatibility of Cinnamomi ramulus the acute toxicity of the experimental results, which proved the acute toxicity of compatibility with the main role for antagonist, In the same effect, compatibility of the strength of the antagonist group size:Herbal ephedrae-Cinnamomi ramulus1:0.5group<Herbal ephedrae-Cinnamomi ramulus1:1group <Herbal ephedrae-Cinnamomi ramulus1:2group. Along with the increase of the content of Cinnamomi ramulus. Herbal ephedrae and Cinnamomi ramulus compatibility of acute toxicity antagonist obviously increased.5、Herbal ephedrae-Cinnamomi ramulus injection of yeast-induced rat fever with antipyretic effects caused by the yeast model of rat fever. Herbal ephedrae-Cinnamomi ramulus1:0.5group, Herbal ephedrae-Cinnamomi ramulus1:1group,Herbal ephedrae-Cinnamomi ramulus1:2group and all the party group multiple comparison was no significant difference(P>0.05).6、Processing the by Calcusyn software judgment drugs the relationship between the CI value CI <1, so the combination of Herbal ephedrae-Cinnamomi ramulus synergistic effect.7、The aconitine zebrafish embryos to have heart toxic effect. Ephedrine and aconitine compatibility, along with the increasing of the concentration of methamphetamine, zebrafish embryonic cardiac toxic increase. |
PDF Full Text Request