Quantitative Expression And Clinical Significance Of HTERT MRNA And MUC4mRNA In Peripheral Blood Of Patients With Pancreatic Cancer

Objective To study the quantitative expression and clinical significance of hTERT mRNA and MUC4mRNA in peripheral blood of patients with pancreatic CancerMethods Real-time fluorescence quantitative RT-PCR method was applied to investigate the quantitative expression of hTERT mRNA and MUC4mRNA in peripheral blood of patients with pancreatic Cancer and healthy controls.Results The expression levels of peripheral blood hTERT mRNA and MUC4mRNA in patients with pancreatic Cancer were significantly higher than those in normal controls(7.95±5.46vs.0.92±1.07,P<0.01;38.25±25.07vs.4.37±5.96, P<0.01). The expression of hTERT mRNA was associated with lymph node metastasis, surrounding organ infiltration or far metastasis and TNM staging of pancreatic cancer patients(P=0.036;0.027;0.019). The expression of MUC4mRNA was associated with lymph node metastasis, surrounding organ infiltration or far metastasis and TNM staging of pancreatic cancer patients(P=0.041;0.022;0.017).Conclusion Abnormal expression of hTERT mRNA and MUC4mRNA correlates intimately with infestation and metastasis in pancreatic cancer, and hTERT mRNA and MUC4mRNA from peripheral blood cells could be effective tumor markers for pancreatic caner.