Effects Of Three Extract Punica Granatum L. On Cell Proliferation，in3T3-L1Preadipocytes

Object: The project breakthrough point is to improve the fat cell proliferation. In thisresearch，the object of observation as classic3T3-L1preadipocytes, On the one hand，to seekthe cell culture of3T3-L1preadipocyte optimal conditions and accumulation of cultureexperience; On the other hand, the effects of SPEAE（ethyl acetate extract sour fruit ofPomegranate）、SPEE（ethanol extract sour fruit of Pomegranate）、SPAE（50%acetone extractsour fruit of Pomegranate）and TPEAE（ethyl acetate extract sweet Pomegranate）、TPEE（ethanol extract sweet Pomegranate）、TPAE（50%acetone extract sweet Pomegranate）underthe stimulation of IGF-1and TNF-，on the3T3-L1preadipocytes were researched. Effectof the medicine on the proliferation and morphology,Preliminary study the drug (pomegranate)on fat cell proliferation occurs in the development of improved，And to explore its roledose-effect and time-effect situation. Basing on the findings，the possible mechanism was alsoexplored.Methods:1,3T3-Ll preadipocytes proliferation culture experiment；2、The intervention effect of SPEAE、SPEE、SPAE and TPEAE, TPEE, TPAE on the3T3-Llpreadipocytes;3、The intervention effect of SPEAE、SPEE、SPAE and TPEAE, TPEE, TPAE on the3T3-Llpreadipocytes with IGF-1；4、The intervention effect of SPEAE、SPEE、SPAE and TPEAE, TPEE, TPAE on the3T3-Llpreadipocytes with TNF-.Result:1、 Normal3T3-Ll preadipocytes were identified and cultured for further experiments.2、 Different mass concentrations of SPEAE、SPEE、SPAE and TPEAE, TPEE, TPAE canPromotes the proliferation of3T3-Ll preadipocytes in a dose-dependent and time-dependentmanner.①、24h, SPEAE, TPEAE increased concentration of quality, promote the proliferationobviously increase, better than48h and72hours; And the single dose20μg/mL~100μg/mL between, the same quality of the concentration of the SPEAE, TPEAE, with24h~48h, present must restrain proliferation role；②、SPEE, TPEE and SPAE, TPAE effect time24h,48h are significantly promote the role ofproliferation,72h effect is not obvious.3、concentrations IGF-1under the stimulus of3T3-L1before fat cells present obviousproliferation role; The experiment of selected set concentration of igf-ii,1stimulate3T3-L1former fat cells, different quality of the concentration of the SPEAE, SPEE, SPAE andTPEAE, TPEE, TPAE are significantly promote the total sum of the proliferation of the role,the role of efficiency and limitation on relations：①、24h, as SPEAE, TPEAE increased concentration of quality, promote the proliferationobviously increase, better than48h and72hours; And the single dose20μg/mL~100μg/mL,the same quality of the concentration of the SPEAE, TPEAE, with24h~48h, present mustrestrain proliferation role；②、SPEE, TPEE and SPAE, TPAE effect time24h,48h are significantly promote the role ofproliferation,72h effect is not obvious.4、concentrations TNF-for3T3-L1fat cell proliferation before the present obviousinhibition; The experiment of selected set concentration TNF-proliferation of suppressing3T3-L1former fat cells, different quality of the concentration of the SPEAE, SPEE, SPAEand TPEAE, TPEE, TPAE display against proliferation inhibition and promote the role of theproliferation, present concentration-response and aging relationship；①、24h in time, SPEAE, TPEAE shows proliferation is obviously promote role, but the roleis weak in promoting proliferation TNF-intervention the strength of the role;20μg/mL to100μg/mL interval, single doses of SPEAE, TPEAE in24h~48h time, along with theextension of time, the inhibition effect on a proliferation; In48h time points, SPEAE,TPEAE along with the increase of concentration, all showed that inhibit the proliferation ofaction；②、SPEE, TPEE proliferation of promoting role time,24h better than48h,48h is better thanthe72h. That role with the extension of time, promote the proliferation action is reduced；Conclusion: acid pomegranate, sweet pomegranate extract on different3T3-before theproliferation of fat cells l1intervention basic similar role. Among them, the sweetpomegranate promote proliferation role in the role of weak acid pomegranate, this may be asthe former less than the latter the sugar content. Acid pomegranate and sweet pomegranateethyl acetate part, single dose display obvious role as time increases the inhibition ofproliferation role; While acid pomegranate and sweet pomegranate ethanol part and50%acetone part all showed that promote proliferation role, this two parts to the polarity extract,main sugar, thus promoting role performance proliferation.