| Molecularly Imprinted Polymers(MIP) are specific high Polymer materials thathave the selective binding ability to the particular molecules. MIP involveco-polymeirzation of functional monomer molecules with cross-linking agents in thepresence of template molecules to create three dimensional network polymers. Then,by chemical methods removal of the template molecules yields vacant recognitionsites complementing both chemically and sterically to the impirnting moleculestructure. Because of the molecularly impirnted polymers have the advantages ofspecificity, practicability, predetermination, better stability, as well as long life andwide range of applications. They have been extensively used in more ifelds such aschromatographic separation, solid phase extraction，environmental monitoirng, catalysreaction and other relevant ifelds. But the traditional preparations have manydisadvantages, such as，it is very difficult to completely eluting templates rfomMIPMs，the small binding capacities to templates, bad accessibility,and as well asresulting in the determination accuracy. So，in recent years, there appears some newmolecular impirnting technologies to solve the disadventage. Such as suspensionpolymeirzation, surface impirnting technique, single-step swelling and polymeirzationand so oruIn this study，Osthole and salidroside were firstly chosen as template molecule,the single-step swelling and polymeirzation was developed to synthesize MIPMs inpolar solvent. Here, the unique concave-dipression spatial structure has successfullybeen prepared. The surface morphology of the MIPMs was characteirzed by scanningelectron microscope. The adsorption and combine properties were characteirzed byUV. The prepared MIPMs were used as the stationary phase of Solid Phase Extraction(SPE) cartirdges and the conditions were optimized. And the imprinting,mass transfer,recognitionand and adsorption mechanism of Osthole and salidroside MIPMs werefurther investigated.The contents of this thesis include the following aspects: (1)Using the polystyrene particles as seeds, adopting appropirate swelling andpolymeirzation techniques, Osthole and salidroside were chosen as template molecule,the MAA and AM as the functional monomer separately, DMF as the solvent,monodisperse MIPMs were prepared successfully by a single-step swelling andpolymeirzation method, the microspheres were uniform and had good dispersibilitywith5|j.m in diameter. It was excited to ifnd that the obtained microspheres had lots ofconcave-dipression or cracks. The factors affecting particle size and morphology ofMIPMs were studied. It was found that the different cosslinker dosage played vitalrole on the particle size of MIPMs. It was also found that the solvent and the swellingtime played vital role on the morphology of MIPMs. The MIPMs were characterizedwith scanning electronic microscope.(2)Studying on the combination of template polymer dynamicts, and the dynamicadsorption of osthole molecular impirnting polymer, the selective bindingcharacteirstics of the template polymer were evaluated by Scatchard analysis. Theresults showed that: the groove surface of synthetic microspheres increased surfacearea and binding sites, effectively raising the rate with the combination of osthole and“impirnted capacit"y. Dynamic adsorption of osthole molecular impirnting polymerIndicated adsorption increased quickly and reached saturation in a short peirod of timeabout50min. It was studied the only one selective sites in osthole-MIPMs by staticadsorption expeirments and Scatchard analysis, the dissociation constant Kd andmaximum apparent adsorption quantity Qmax of these binding sites are6.52mmol/Land93.68[imol/g，respectively. The prepared Osthole-MIPMs were used as thestationary phase of Solid Phase Extraction (SPE) cartirdges and the conditions wereoptimized. The results show that Osthole-MIPMs exhibited higher selectivity andrecognition ability for Osthole than non-impirnted polymer (NIPMs). the applicationof Osthole-MIPMs with high affinity and excellent stereo-selectivity toward ostholein SPE might offer a new method for the enrichment and determination of ostholecompounds in the natural products.(3)The Salidroside-MIPMs were characteirzed with scanning electronicmicroscope, the adsorption properties and adsorption mechanism were inventigated.Meanwhile, choosing structurally similar compounds of Polydatin was empoiyed formolecular recognitor*specificity tests of Salidroside-MIPMs.The salidroside-MIPMswere used as a solid phase extraction sorbent, The MIPMs were charaterization in theterm rebinding property with analyte, selectivity and extraction of target analyte from Salidroside or Salidroside and Polydatin mixture solution. The extraction and enrichof Salidroside in Rhodiola rosea samples followed by HPLC analysis. Theexpeirmental results showed that the Salidroside—MIPMs exhibited a favorableaiffnity for Salidroside because their binding sites can be accessed more easily. Theadsorption kinetics was shown that the Q almost reached equilibrium after40min.The saturated binding capacities Qmax obtained for Salidroside-MIPMs was98.52jimol/g. The value of K was0.1432U jamol in Langmuir adsorption analysis. Theseparation factor a=1,53, The Salidroside-MIPMs exhibit good recognition propertiesfor the Salidroside. After optimization of SPE profile, it was found that the bestimpirnting effect was obtained with loading in methanol, washing with5mLmethanol-HiO (5:95，V/V)，eluting with5mL methanol-H20(10:90，V/V),... |
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