MicroRNA Gene Expression Deregulation And Functions In Laryngeal Squamous Cell Carcinoma

Objective In order to screen out the differentially expressed miRNA in laryngeal squamous cell carcinoma and to understand what’s the roles they play in tumor procession.Methods The miRNA expressing profiles consisting of210miRNA probes and the mRNA expressing profiles consisting of7267genes of Laryngeal Carcinoma and normal tissues were compared, hierarchical clustering was used for the gene expression, and parts of the results were confirmed through semi-quantitative RT-PCR。At the same time, dueing to miRNA being blocked in HEp-2, the level variations of mRNA and protein were detected by RT-PCR and Western blot. Then, the target genes of miRNA predicted by bioinformation were confirmed by EGFP reporter system. At last, MTT assay, Anchorage-independent assay and Cell cycle analysis were used to analyze the miR-21effects on growth and proliferation of HEp-2. We took use of Aggregation Assays, Monolayer wound assays and Transwell migration assay to analyze the miR-16influence in the cell-cell adhesion and cell migration of HEp-2.Results In this research, we found13miRNA expressed differentially in Laryngeal Carcinoma, with6up-regulated and7down-regulated。In mRNA expression profiles,90genes were expressed repeatedly and differentially in3cases of Laryngeal Carcinoma, Among these genes, up-regulated were29and down-regulated were63。At the same time, we found that ASO-21could increase the level of BTG2mRNA and release the repression of miR-21on EGFP expression in EGFP reporter system, and it also could decrease the activities and proliferation capacity of HEp-2, repress the transition from G1to S of the cell cycle. In addition, ASO-16could increase the ZYXIN expression in both mRNA level and protein level, release the repression of miR-16on EGFP expression in EGFP reporter system and enhance the capability of cell-cell adhesion, inhibit the migration of HEp-2.Conclusion Microarray were used to screen out the differentially expressed miRNA and mRNA in laryngeal squamous carcinoma。miR-21could increase the activities and proliferation capacity of HEp-2by targeting BTG2. miR-16could reduce the capability of cell-cell adhesion and enhance the migration of cell by targeting ZYXIN。In this research, it indicated that miRNA took part in the progression of laryngeal carcinoma. And it was helpful for us to understand the mechanism of laryngeal carcinoma biogenesis and development.