Gene Expression Analysis And Prognostic Significance Of Stefin A And Cathepsin B In Laryngeal Squamous Cell Carcinomas

OBJECTIVETo investigate the expression of the Cysteine proteinase cathepsin B and its endogenous inhibitor stefinA in laryngeal squamous cell carcinomas (SCC) and the relationship between their expression, the laryngeal biological behavior and the Prognostic significance. METHODSThe gene expression of Cysteine proteinase cathepsin B and its endogenous inhibitor stefinA in fresh laryngeal cancer tissues and their corresponding normal mucosas was detected by RT-PCR. The expression of cysteine proteinase cathepsinB and stefinA proteins in paraffin-embedded tissues was determined immunohistochemically by means of tissue microarray (TMA).All statistical analyses were performed by SPSS version 11.5. RESULTS(1) In the 41 cases of matched specimens, cathepsin B gene expression was much higher in tumor tissues than that in corresponding normal tissues(P<0.05). Whereas there is no significant difference (p>0.05) between the tumors and the corresponding normal tissues for stefin A.