The Expression And Significance Of ILK In Laryngeal Squamous Cell Carcinoma On Tissue Microarray

Laryngeal carcinoma is one of most common malignant neoplasm inhead and neck. In recent years, the morbidity of laryngocarcinomaappears an increasing tendency. It does great harm to people's health. Ithas become special focus to investigate the oncogensis, developing,metastasis and management of the tumor from the molecule level. A newviewpoint is cell signal transduction pathway abnormality and cellabnormal growth, generation and differentiation signal induce cellcancerization.Integrin -linked kinase (ILK) is a serine/threonine kinase that hasimplicated in integrin, growth-factor, and Wnt signaling pathway. ILKparticipates in the regulation of cell survival, proliferation, differentiation,adhesion, migration and so on. ILK was proved to be a cancer gene inmany investigations. ILK participate many processes of tumorigenesis,and connect with tumor cell generation, adhesiveness, migration andvascular formation. Since the crucial role of ILK in transmittingintracellular and extracellular signals, as well as the cell cycle arrest andthe initiation of cell apoptosis by suppressing the activity of ILK, ILKbecomes an attractive target for tumor gene and medicine therapeutics.Tissue microarray is a new method followed with the development ofgene microarray. It can high efficiently detect clinical tissues, has many characteristics, such as fastness, convenience, economy.Objective: To study the methods of preparation tissue arrays andapply the results of application to immunohistochemistry(IHC). Toinvestigate the expression of integrin-linked kinase(ILK) in laryngealsquamous cell carcinoma and normal tissues adjacent to cancer, and studythe correlation between its expression and the clinical-pathologicalparameters of primary laryngeal squamous cell carcinoma.Method: Paraffin-embedded tissues from 36 human laryngealcancer samples and 18 normal tissues adjacent to cancer made tissuesmicroarray paraffin according to predesign. Tissue microarrays paraffinwere used to cut and toast, and made to tissues chip. Expressional level ofILK was detected in 36 human laryngeal cancer samples and 18 normaltissues adjacent to cancer respectively, and their correlation with lymphnode metastasis and the degree of call differentiation were studied byimmunohistochemistry(IHC) methods on tissue microarray. The statisticalevaluation was performed with SPSS software for windows.Results:1. Middle density tissues arrays were prepared with this methods andorderliness of range, outlook of shape, uniform of the tissue dots and notmissing on the slide. Good results were obtained in HE stain. Tissuemicroarrays were used to immunohistochemistry, conveniented toobserve and diagnosis. 2. The expressions of ILK in LSCC are obviously higher than that innormal tissues adjacent to cancer and there was statistical significance (P＜0.01).3. The expressions of ILK in well-differentiated samples were lowerthan moderately and poorly differentiated samples, and there wasstatistical significance (P＜0.05).4. The expressions of ILK in LSCC with lymph node metastasis wereobviously higher than that without, and there was statistical significance(P＜0.01).5. The positive rates of ILK inⅠ—Ⅱstages andⅢ—Ⅳstageswere 66.67％(8/12) and 70.83％(17/24), there was no statisticalsignificance (P＞0.05). The positive rates of ILK in supra-glotticcarcinoma and glttic carcinoma were 72.73％(8/11) and 66.67％(16/24).There was no statistical significance (P＞0.05).Conclusions:1. Tissue microarray is a relatively well-rounded technology, and hasmany advantages, such as high efficiency, convenience, economy andstandardization.2. Our study results suggest that ILK are closely related to themalignance degree of laryngeal cancer, the degree of cell differentiation.In the LSCC, ILK over expressed, and had relationship with cervicalmetastasis, which showed ILK had a close relationship with LSCC and its metastasis. The study of relationship of ILK and laryngeal carcinomareminds us a new gene therapy of laryngeal carcinoma by inhibiting thefunction of ILK.