Nano Composite In Vitro Study Of Antibacterial

BackgroundBiological materials implanted in the body after the most severe complications areinfection, its ending is devastating, once the cause of treatment failure.After infection,implanted biological material has its own characteristic and pathogenesis, its basic reasonlies in the biomaterial surface bacterial biofilm formation.In the animate or inanimateobjects surface bacteria, bacterial own secretion is highly organized and multicellularcommunities structure and containing extracellular mucous substance structure called thebacterial biofilm.In the inner of the bacterial resistance to outside intrusion and avoidanceof therapeutic effects of antibiotics, bacterial biofilm is the leading cause of biomaterialimplant infection is difficult to control root, bacterial biofilm formation environmentcorresponding planktonic cells form, which is composed of bacterial extracellularpolymers.Bacterial growth protection, can be connected to biomaterial implant is formedabove has very strong density organized multicellular structures.Biological material, on thebiofilm structure and functional characteristics are: bacterial attachment to a surface of thesupport for the existence of colonization is favorable, more conducive to resist the hostimmune cells and molecules, antibacterial drugs against compared with single orsuspension of bacterial cells, and also increases the resistance genes and Toxicology Genetransfer.It is difficult to eliminate the root cause is the structural and functionalcharacteristics.Biological membrane treatment at present there is no satisfactory clinical outcomes,there are sound splitting method, ultrasonic method, eddy current method, electricalstimulation, both at home and abroad there is no reliable suppression and preventionmeasures.Some implants replacing also invalidate some planktonic bacteria resistantstrains had no effect, and may be caused by drug-resistant strains of infection.Nano silverbroad antibacterial spectrum, strong antibacterial activity, and is an ideal antibacterialcoating materials, coating to produce sterile antibacterial materials applied in biomaterialsurface provides a feasibility study of the new ideas. Part OneObjective：Preparation of nanometer silver antibacterial coating containing.Detection of silvernano composite featuresMethods:According to the mass ratio of1:20ratio powder average particle straight10mhydroxyapatite and5u m powder preparation.Fully and uniformly dispersed in two kindsof powder, the powder in type two QM3SP2(Shanghai planetary ball mill is mixed30minutes).Application of HH-ZK type constant temperature water bath in the mixed powderon titanium substrate by electrophoretic deposition to.Detection and characterization ofcoating compositions using X-650scanning electron microscope, D/MAX2550VB/PCtype X ray diffraction and Magna-IR type550FTIR spectrometer.Results：Particle size shows an average of about100nm of nano silver particles can bedispersed and hydroxyapatite powder mixed fully through SEM, FTIR and XRD analysis Part TwoObjective:Test of Staphylococcus epidermidis in biofilm morphology and adhesion capacity,through the nanometer silver loaded implant surface on the biofilm formation ofStaphylococcus epidermidis effect.Material and method:1.Experimental strains for positive Staphylococcus epidermidis.In37similar tohuman environment environment will be sterile silver nanometer composite coatingsamples and sterile excluding silver composite objects are put into1.0x107CFU/ml10mlsuspension culture.In6,24,48,72hours of silver nano composite samples and does notcontain silver composite surface biological membrane by confocal laser scanningmicroscopy on biological membrane morphology and surface biofilm bacteria count andcontrol.2.The prepared sample composite coating in fetal bovine serum using ICP-NS(Agiilent) to detect and to test its release rate, at the same time the sample method of thesame are placed in the air after12weeks to detect.Simultaneous measurement ofStaphylococcus epidermidis adhesion molecule AtlE expressionResult:Does not contain silver composite objects in12hours has been the emergence ofmature biofilms of Staphylococcus epidermidis, silver nanometer composite surface48small until after the emergence of Staphylococcus epidermidis biofilm, at the same timebetween the two groups in the surface coating adhesion of bacteria amount difference hasstatistics significance (p<0.01).Conclusion:Ag/HA composite coating obviously inhibited biofilm formation. The coating loadedwith silver obviously changed the surface activity of the coating, and thus bacterialadhesion decreased.