Effect Of Oriented Nano-silk Protein On The Morphology And Proliferation Of Bone Marrow Mesenchymal Stem Cells In Vitro

Background and Significance:Oriented nanostructures which can affect cell morphology, migration, proliferation and differentiation have an important impact on cell biological behavior. However the role of oriented nano-silk in inducing bone marrow mesenchymal stem cells(BMSCs) osteogenic differentiation has not been reported. The treatment of large bone defects remains a challenge to orthopedic surgeons. It is to investigate orientated nano-silk protein’s effect on the differentiation, proliferation and osteogenic differentiation of BMSCs.Objective:The purpose of this study is to investigate whether the oriented nano-silk protein can promote BMSC proliferation and osteogenic differentiation in vitro.Methods:Oriented and random Nano-silk protein can both be produced by electrospinning with different rotation speed. BMSCs were cultured on the surface of the materials. Cell morphology and structures of the cytoskeleton were observed under microscope and electron microscope. With MTT assay, the influence of nano-silk protein on cell proliferation was also investigated. Results:The diameter of silk ranged between500nm and1000nm. BMSCs which were cultured for3days on oriented nano-silk were narrower and more aligned and stretched out longer synaptic to adhere to the surface of the silk fiber compared with those on random-silk. It was also observed that BMSCs which were cultured on oriented nano-silk had a higher proliferate rate than those on smooth silk after3days, and higher than those on random silk after4days.Conclusion:Orientated nano-silk was capable of inducing BMSCs to aligned and promoted BMSCs proliferation.