| Objective：To establish an animal model of congestion on liver partly inrats based on partial liver resection, and using two different methods to dealwith the congestion：keep and resection, observing and comparing the effectof liver regeneration and function after partial liver resection in rats of earlystage in the two models.Methods: First step, to establish rat animal model:①30rats were usedfor liver anatomy study to learn about the anatomy of the rat liver;②Toestablish an animal model of reserves the right liver lobe of rats (75%liverresection) plus blood congestion of the anterior of caudate lobe anterior blood.Two different treatments were conducted on the basis of this model:39adultmale SD rats were randomly divided into2groups: group A(n=20), the bloodcongestion lobe was reserved; Group B(n=19), the blood congestion lobe wasremoved after3minutes of congestion. The second step was to compare theinfluence of early liver regeneration and liver function between the twogroups:①rats were sacrificed after different postoperative time points (1d,2d,3d,5d) for blood and liver specimens collection.②the relevant indexdetection: postoperative serum ALT, AST levels, and assessment of theseverity of the Postoperative liver function; Pathology were observed withrHE staining, light microscope to observe different liver pathological changes;HGF and TNF-a were detected by ELISIA method.Results:Step1: Do partial liver resection in rats within the scope of security, two models between keep and resection the congestion were stability, suitable forrelated research.Step2:1. Liver regeneration:(1).liver regeneration index:①the volumeratio of the right liver lobe were gradually increasing day by day and group Awas obviously higher than group B (P <0.05) at postoperative days1,2, butthere was no statistical difference (P>0.05) at fifth day.②The ratio of liverweight and body weight, group A were significantly higher than that of groupB in postoperative1,2,3,5days (P <0.01).(2) the TNF-a level: the level ofTNF-a reached a peak in2days both in two groups, and group A issignificantly higher than group B at postoperative days1,2(P <0.05), but theresults were no statistical difference in postoperative day3,5day (P>0.05).(3) the HGF levels: the serum HGF level in both groups peaked onpostoperative day1, then gradually decline, and group A was significantlyhigher than group B (P <0.05) at postoperative day1,2day, but no statisticaldifference at day3,5(P>0.05).2. The pathological changes: thenon-congestion lobe was different proportions of degeneration atpostoperative1,2,3days in both groups, and returned to normal at fifth day;Congestion areas of liver lobe was congested significantly and some areas ofpatchy necrosis and hepatocellular fatty degeneration at postoperative days1,2; the liver cell necrosis at postoperative days3,5.3. The liver functionchange: two groups of postoperative serum ALT and AST levels onpostoperative day1reaches its peak, and gradually reduced. The ALT, ASTlevel in group A were significantly higher than that of group B atpostoperative day1, day2,3days (P <0.05); but in both groups showed nostatistical difference (P>0.05) at day5. though, AST level in group A wasstill higher than group B (P <0.05). Conclusions:1. The each lobe of rat liver was independent, do partial liver resection inrats within the scope of security, two models between keep and resection thecongestion were stability, suitable for related research.2. Contrast to remove congestion after liver resection, keep congestion ofliver tissue have a positive promoting effect for early liver regeneration, but atthe same time, keep congestion can aggravate the damage of liver function. |
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