Influences Of Laser Treatment On The Expressions Of The Plasma Vascular Endothelial Growth Factor And Basic Fibroblast Growth Factor In Children Cutaneous Hemangioma

Objective and significance:Hemangioma is a common congenital benign tumor of infancy, which is made of abnormal vascular endothelial cell proliferation of vascular tissue. It has obvious growth cycle and could occur in any part of the body. In general, it grows rapidly in one year. The three periods of this disease are proliferating hemangiomas, involuting hemangiomas and involuted hemangiomas. If it could not be controlled effectively in the proliferating period, it may do harm to the patients’ appearance and even threat to their lives.The hemangioma may be caused by the abnormal expression of stimulating factors or inhibiting factors of angiogenesis. Researches suggest that the happening of hemangioma is closely related to the level of VEGF and bFGF in recent years. According to the study of Takahashi, the level of VEGF is high in the endothelial cells of proliferating hemangiomas while it is not expressed in the involuting hemangiomas. According to the research of Bielenbery, the level of bFGF is highly expressed in the vascular tissue of proliferating period which it is normal in the involuting tissue and normal tissue.At present, main methods for the treatment of hemangioma are clinically laser treatment, hormone treatment, radiotherapy, interferon treatment, surgical treatment, etc. Due to the laser treatment is not easy to produce scar, change pigment and not need to be hospitalized and so on, the laser treatment is more and more used to cure infant skin hemangioma. Its principle mainly depends on the selective solar-thermal role.However, for the therapeutic effect of laser treatment, it mainly depends on the history and physical examination while it lacks a simple and reliable objective detection methord.In this study, VEGF and bFGF which is closely related to the angiogenesis are chosen as the research object. The plasma of different periods of infant skin hemangioma with laser treatment are examined. The study is used to investigate the influences of laser treatment on the expressions of the plasma VEGF and bFGF in children cutaneous hemangioma and find a simple and reliable method for the evaluation of laser treatment.Methods:109infant hemangioma patients with laser treatment were engaged in this study, including74proliferating phase patients,20involuting phase patients and15involuted phase patients,10non-hemangioma patients were selected as the control. The blood plasma of all the patients was selected for the further text. The plasma levels of VEGF and bFGF were measured with the enzyme-linked immunosorbent assay (ELISA). The plasma levels of VEGF and bFGF of23proliferating phase patients were dynamically observed before and after the treatment. The data were analysed with SPSS16.O software package.Results:The plasma VEGF concentrations in proliferating hemangiomas were different from that in the involuting hemangiomas, involuted group and negative control, while the differences between the latter three groups were not significant. The plasma bFGF concentrations of proliferating hemangiomas were significantly higher than those of the latter three groups, while the differences between the latter three groups were not significant. After laser treatment, the plasma VEGF and bFGF levels decreased significantly.Conclusion:1.The plasma VEGF and bFGF concentrations decrease after the laser treatment in infant hemangiomas.2. VEGF and bFGF may be the objective index to judge the effect of the laser treatment on infant hemangiomas.