Studies On Chemical Constituents From Berries Of Physalis Pubescens L.

Physalis pubeacens L. has long been a minor fruit of China and has also been grownin America. The fruit of the Physalis Genus was also extensively used as herb medicine,and had the effects of detoxification and anti-inflammation in traditional Chinese medicine.the extract of fruits of the Physalis Genus had been reported to have potent antioxidant,anti-inflammatory, and cytotoxic activities. Previous studies on constituents from thePhysalis genus led to the isolation of different compounds such as triterpenoids, flavones,alkaloids and steroids.During the course of our investigation for bioactive agents from the EtOH extract ofcalyxs of Physalis pubeacens L.,30compounds were obtained by using silica gel columnchromatography, Sephadex LH-20chromatography, MPLC, and Semi-HPLC, andstructures of26compounds were elucidated through spectroscopic analysis (NMR, MSand IR). Their structures were identified to be3,7,3’-trimethylquercetin (SG-1),kaempferol (SG-2), chrysoeriol (SG-3), quercetin-3-O-β-D-glucopyranoside (SG-4),arjunolic acid (SG-5), pulsatilla saponin A (SG-6), pulsatilla saponin D (SG-7),blumenolA (SG-8), caffeic acid (SG-9),1-O-caffeoyl-β-D-glucopyranoside (SG-10),syringic acid β-D-glucopyranosyl ester (SG-11),5-O-caffeoyl quinic acid butyl ester(SG-12),1-p-coumaroyl-β-D-glucopyranoside(SG-13), Calceolarioside A(SG-14),4-hydroxyl-3-methoxyl-benzoicacid(SG-15),1-O-p-feruloyl-β-D-glucopyranoside(SG-16), N-trans-p-hydroxyphenethylferolamine (SG-17), neo-olivil (SG-18),medioresinol (SG-19), pinoresinol (SG-20), sucrose (SG-21), uridine (SG-22), adenosine(SG-23), northrachelogenin (SG-24), β-sitosterol (SG-25), daucosterol (SG-26)。Amongthem, SG-5~SG-8,SG-10,SG-11,SG-13~SG-24were isolated from this genus for the firsttime. Compounds SG-3,SG-4and SG-12were obtained from this plant for the first time.The antibacterial, antioxidation and antitumor activitis of compounds and extract were screened, SG-20at a dose of0.016μmol/mL-0.256μmol/mL,SMMC-7721hepatomacell inhibition was23.58%-49.21%. SG-11at a dose of0.0087μmol/mL-0.0348μmol/mL,A549lung cancer cells inhibition was10.66%-26.86%.SG-20at dose of0.0022μmol/mL-0.0344μmol/mL,A549lung cancer cell inhibition was22.74%-52.48%..ABTS free radical clearance of70%methanol parts of the berries was16.95%-91.53%,when the concentration was between0.0625mg/mL–4mg/mL, ABTS freeradical clearance of50%methanol parts of the berries was3.39%-71.19%,when theconcentration was between0.0625mg/mL–4mg/mL, ABTS free radical clearance of30%methanol parts of the berries was1.69%-67.80%,when the concentration wasbetween0.0625mg/mL-4mg/mL. ABTS free radical clearance of70%methanol parts ofthe berries was26.35%-92.96%,when the concentration was between0.0625mg/mL~4mg/mL,Sort of clearance was the alcohol extracts>70%methanol parts>50%methanolparts>30%methanol parts. DPPH free radical clearance of70%methanol parts of theberries was12.89%-90.42%,when the concentration was between0.0625mg/mL-4mg/mL, DPPH free radical clearance of50%methanol parts of the berries was5.13%-68.80%,when the concentration was between0.0625mg/mL-4mg/mL, DPPH free radicalclearance of30%methanol parts of the berries was4.32%-65.77%,when theconcentration was between0.0625mg/mL–4mg/mL. DPPH free radical clearance of70%methanol parts of the berries was26.35%-92.96,when the concentration was between0.0625mg/mL~4mg/mL,Sort of clearance was the alcohol extracts>70%methanol parts>50%methanol parts>30%methanol parts.The scope of bacteriostatic ring was4.0mm-5.9mm,when the berries of Physalispubeacens L.ethanol extract concentrationwas between12.5mg/mL-100mg/mL,and thePseudomonas aeruginosa was106CFU/mL. The scope of bacteriostatic ring was4.0mm-6.2mm, when the berries of Physalis pubeacens L.ethanol extract concentrationwasbetween12.5mg/mL-100mg/mL,and the Staphylococcus aureus was106CFU/mL.Thescope of bacteriostatic ring was4.0mm-5.3mm, when the berries of Physalispubeacens L.ethanol extract concentrationwas between12.5mg/mL-100mg/mL,and theStaphylococcus aureus was106CFU/mL, The scope of bacteriostatic ring was4.0mm-6.1 mm,when the berries of Physalis pubeacens L.ethanol extract concentrationwas between12.5mg/mL-100mg/mL,and the Bacillus subtilis was106CFU/mL. The scope ofbacteriostatic ring was4.0mm-6.4mm.,when the berries of Physalis pubeacens L.ethanolextract concentrationwas between12.5mg/mL-100mg/mL,and the E. coli was106CFU/mL.This study provided research data for natural medicine and modern Chinese medicinepharmacological,in order to achieve the purpose to rational drug use,and broaden its scopeof application, And it could be employed as natural antioxidants and health care products.