| Background and objectiveCervical cancer is one of the most common cancers in women worldwide, especially in developing countries,which caused about35million deaths per year. The people who have it get younger and younger. Cervical cancer which is one of important cancer prevention in China had threatened to fertility and health of women,and it poses an important public health problem. Approximately80%of primary cervical cancers arise from pre-existing squamous dysplasia. The most important etiologic agent in the pathogenesis is high risk human papillomavirus (HPV). However, the people who are HPV-negative also got cervical cancer.This suggests that other cofactors must be present in the pathogenic pathway between cervical dysplasia and carcinoma.The discover of microRNA which in the post-transcriptional level regulate the expression of gene provides new ideas for the pathogenesis of cancer.It is very significantly to improve the diagnosis and treatment of cervical cancer in studying the molecular mechanism deeply, seeking molecular markers for early diagnosis and finding effective therapeutic targets.microRNAs are a class of about19-25nt single-stranded non-coding RNA,which negatively regulate the translational efficiency and stability of target mRNAs. Altered miRNAs expression in disease has been observed, especially in cancer.The deregulation of miRNAs may play an important role in the occurrence and development of cervical cancer. miR-143located in chromosome5q32. miR-143is downregulated in a variety of malignant tumors. miR-143in the diagnosis and treatment of cervical cancer may have potential applications. Although miR-143in cervical cancer cell lines showed low expression, but there is not yet discovered the expression of miR-143in normal cervical tissue, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma. This research aimed to study the expression level of miR-143in normal cervical tissue, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma,and provide a theoretical basis for study of miR-143in cervical squamous cell carcinoma.Materials and Methods1. The experimental group1.1The case group:According to the International Federation of Gynecology and Obstetrics (FIGO) staging system,30samples of fresh cervical squamous cell carcinoma tissues come from the Second Affiliated Hospital of Zhengzhou University between February2012and November2012, which consist of Ⅰ20samples, Ⅱ10samples.30samples of fresh cervical intraepithelial neoplasia (CIN) tissues were collected in the same time, which consist of CIN Ⅰ14samples, CIN Ⅱ-Ⅲ16samples.1.2The control group:30samples of normal cervical tissues due to uterine fibroids hysterectomy were collected in the same period as a control.1.3The inclusion criteria:①All the samples have not been treated by radiotherapy and chemotherapy;②All cervical tissues were examined by pathological diagnosis as cervical squamous cell carcinoma or cervical intraepithelial neoplasia tissues;③The patients or thire family all agreed with the samples selection and signed informed consent.2. The experiment methodReal-time fluorescence quantitative polymerase chain reaction (qRT-PCR) method was used to detect the relative expression of miR-143in the tissues of normal cervical, CIN and cervical squamous cell carcinoma.With U6as an internal reference. The date were analyzed by SPSS17.0program. The measurement data were expressed as standard deviation(x±s) and tested by independent samples T-test or One-Way ANOVA.Inspection level is α=0.05. P<0.05is the level of significant difference.Results1.The average relative expression of miR-143in normal cervical tissues, CIN tissues and cervical squamous cell carcinoma tissues were1.02±0.15、0.93±0.17、0.32±0.23, the difference was statistically significant (F=122.097, P<0.05).Comparing the pairwise comparisons with LSD statistical methods,between normal cervical tissues and CIN tissues the difference was not statistically significant (P>0.05),between cervical squamous cell carcinoma tissues and normal cervical tissues and CIN tissues the difference was statistically significant (P<0.05).2.The average relative expression of miR-143in the high-risk HPV positive and negative were0.87±0.27,0.90±0.24,the comparion between the two groups was not statistically significant difference(t=0.453,P>0.05).3.The average relative expression of miR-143in Cervical squamous cell carcinoma pathological stage G2and G3were0.38±0.47and0.15±0.33, the comparion between the two groups was not statistically significant difference (t=2.742,P<0.05)4.The average relative expression of miR-143in cervical squamous cell carcinoma with positive and negative lymph nodes were0.17±0.40and0.37±0.47, the comparion between the two groups was not statistically significant difference (t=2.506, P<0.05).5.The average relative expression of miR-143in cervical squamous cell carcinoma with tumor diameter≥2.5cm and<2.5cm were0.22±0.06,0.36±0.48, the comparion between the two groups was not statistically significant difference (t=1.709, P>0.05).6.The average relative expression of miR-143in cervical squamous cell with interstitial infiltration depth≥2/5and <2/5were0.31±0.44,0.33±0.89, the comparion between the two groups was not statistically significant difference (t=-0.269,P>0.05).7.The average relative expression of miR-143in cervical squamous cell carcinoma FIGO Ⅰ and Ⅱ were0.36±0.54,0.24±0.41, the comparion between the two groups was not statistically significant difference (t=1.455, P>0.05).8.The average relative expression of miR-143in cervical squamous cell carcinoma with the young(≥40)and the old (<40) were0.31±0.52,0.33±0.57, the comparion between the two groups was not statistically significant difference (t=0.249, P>0.05).Conclusions:l.The low expression level of miR-143are significantly associated with cervical squamous cell carcinoma.2.The expression level of miR-143are not significantly associated with high risk HPV infection.3.The expression level of miR-143in cervical squamous cell carcinoma are significantly associated with pathological stage, lymph node metastasis;but not associated with tumor size, depth of stromal invasion, clinical stage, and age. |
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