Expression Of P63and IKKbeta In Cervical Cancer And Its Significance

Purpose:To detect the expression of p63and IKKβ in squamous cell carcinoma of cervix paired with adjacent normal tissue,and to explore its significance.Methods:We measured the mRNA expression of TAp63and△Np63in a series21cervical squamous cell cancer paired with adjacent normal tissue by quantitative real-time polymerase chain reaction. The expression of P63、ANp63and IKKβ protein was detected in a variety of36cases of cervical squamous cell cancer paired with adjacent normal tissue by immunohistochemistry.Results:1. The relative level of TAp63and△Np63mRNA in the tissue of cervical squamous cell cancer was0.156±0.040and0.264±0.067, respectively, which were markedly higher than those in adjacent normal tissues was0.061±0.019and0.032±0.008, respectively,and there were significant differences between two groups (P<0.05).2. The ration of the relative level of△Np63mRNA and that of TAp63mRNA in the tissue of cervical squamous cell cancer and adjacent normal tissues was1.801±0.654and0.576±0.173, respectively,and there were significant differences between two groups (P<0.05).3. The expression rate of P63protein in the tissue of cervical squamous cell cancer and adjacent normal tissues was91.7%(33/36)and33.3%(12/36) respectively, there were significant differences between two groups (P<0.05). The expression rate of P63protein in the tissue of cervical squamous cell cancer had no obvious correlation with the tumor size、tumor invision and tumor differentiation(P>0.05).4. The expression rate of△Np63protein in the tissue of cervical squamous cell cancer and adjacent normal tissues was88.9%(32/36)and36.1%(13/36) respectively, there were significant differences between two groups (P<0.05). The expression rate of△Np63protein in the tissue of cervical squamous cell cancer had no obvious correlation with the tumor size、tumor invision and tumor differentiation (P>0.05).5. The expression rate of IKKβ protein in the tissue of cervical squamous cell cancer and para-cancer tissues was100%(36/36) and50.0%(18/36) respectively, there were significant differences between two groups (P<0.05). The expression rate of IKKβ protein in the tissue of cervical squamous cell cancer had no obvious correlation with the tumor size、tumor invision and tumor differentiation (P>0.05).6. The expression rate of P63protein was positively correlated with the expression of IKKβ protein in the tissue of cervical squamous carcinoma and para-cancer tissues(r=0.591,P<0.05).7. The expression rate of ANp63protein was positively correlated with the expression of IKKβ protein in the tissue of cervical squamous carcinoma and para-cancer tissues (r=0.492,P<0.05).Conclusion:1. The expression of△Np63mRNA was much higher than TAp63mRNA in the tissue of cervical cancer, while the former was much lower than the later in para-cancer tissues, so the unbalance between TAp63and△Np63may be potential factor to promote cervical cancer. 2. The expression of P63and△Np63protein were both markedly higher than that in para-cancers tissues,but they had no obvious correlation with the tumor size、invision and differentiation,P63perhaps associated with the occurrence of cervical cancer.3. The expression of IKKβ protein was markedly higher than that in para-cancers tissues,but it had no obvious correlation with the tumor size、invision and differentiation, it perhaps associated with the occurrence of cervical cancer.4. The expression of P63and△Np63protein were both positively correlated with IKKβ in the tissue of cervical squamous carcinoma paired with adjacent normal tissue, P63and IKKβ maybe have synergistic effect to promote cervical cancer.