Expression Of Before And After TACE Treatment Of Rabbit VX2Liver Tumor Tissues

Objective: two different methods to grow through the use of open cultivation andclassic CT puncture, establishing rabbit VX2liver cancer model, for TACE treatmentof animal model, before and after surgery underwent imaging observation.Methods:80New Zealand white rabbits, divided into two groups AB, A groupwithout tumor treatment group, group B underwent TACE treatment of tumorinoculation. The two groups were established in rabbit VX2liver tumor model ofopen and CT puncture tumor injection. The imaging method to observe the planting,the use of MRI continuous scanning to calculate the growth rate of tumor. Group Breceived TACE treatment, changes of liver structure and function were observedbefore and after the operation.Results: compared with1, two inoculation methods: A group success rate was90.6%(29/32), B group success rate was83.3%(30/36), statistical tests for P>0.05, twogroups of model success rate had no significant statistical significance in statistics. Agroup of inoculation time of28.6+4.90, B were inoculated with time14.8+2.48,P<0.05.Detection of2, the growth rate of tumor:17D growth rate of tumor was92.8%,21d was75.3%, compared with p<0.05and14D.Conclusion: the time of inoculation1, modified CT positioning tumor injectionmethod is significantly less than the tumor injection of classic, although the successrate of vaccination was not statistically significant, but the CT positioning withconvenient operation, good experimental animal condition after the operation, is amethod for preparation of rabbit VX2liver cancer model fit.2, the tumor volume andgrowth rate was found at2-4weeks is the most vigorous growth period of tumor.Passage, planting, treatment should not be too late, should be in3-4weeks of rapidgrowth, the most abundant blood supply, solid tumor maximum when the testoperation. Objective: the morphological changes observed by HE staining in rabbit VX2tumortissues and the surrounding normal tissue before and after surgery,immunohistochemical method to detect the expression of NF-, B before and aftertreatment in hepatocellular carcinoma model of TACE tumor tissue andtumor-adjacent tissues, to study the liver TACE tumor treatment group in the controleffects and related mechanism of action.Methods:80New Zealand white rabbits, divided into two groups AB, A groupwithout tumor treatment group, group B underwent TACE treatment of tumorinoculation. Postoperative samples were obtained for HE staining andimmunohistochemistry.Results: the two groups of immunohistochemical detection of NF-, B proteinexpression: A1group29cases of tumors,21cases of positive expression of NF-, B,the positive rate was72.4%, A2positive expression corresponding to11cases, thepositive rate was37.9%; the B1positive expression in24cases, the positive rate was82.4%,18cases of B2positive expression, the positive rate was60%. The two groupand comparison group had no statistical significance (p<0.05).Conclusion: the two group and the group of NF-, B protein expression had nostatistical significance. But NF-, the positive expression of B after TACE tumor tissueand tumor adjacent liver tissue were increased. Description of tumor tissue aftertreatment of cancer cell apoptosis and the surrounding liver tissue injury may lead toincreased expression of NF-, B.