Study Of The Effect Of Nanofiber-peptide Modified Scaffolds For Bone Engineering On Biocompatibility And Osteogenesis

In order to study the effects of nanofiber-peptide modified scaffolds onthe proliferation and osteogenic differentiation of rat adipose-derived stem cells(rADSCs) in vitro, and bone regeneration effects in vivo,nano-Hydroxyapatite/Collagen (nHAC) scaffolds were combined withnanofiber-peptide (cnHAC). The macrostructure and morphology of the scaffoldswere characterized. Scanning electronic microscopy (SEM) showed that thenanofiber-peptide formed a coating on cnHAC scaffolds whose original average poresizes is about300μm with interconnected porous structures. Rat adipose-derivedstem cells (rADSCs) were seeded on nHAC or cnHAC scaffolds and cultured till14days in vitro. ADSCs were observed to adhere to and fully extend on cnHAC byCLSM, which was better than that on nHAC. Based on the results of SEM, DNAcontent, alkaline phosphatase (ALP) activity, and osteogenic gene expression analysis,the responses of rADSCs to the scaffolds exhibited a higher degree of attachment,growth as well as osteogenic differentiation than those on nHAC scaffolds in vitro. Toinvestigate the in vivo biocompatibility and osteogenesis of the composite scaffolds,both nHAC scaffolds and cnHAC were implanted in rat skull bone defect for4w and6w, and then studied radiographically. The in vivo results showed that cnHACscaffolds exhibited good biocompatibility and dramatically enhanced the efficiency ofnew bone formation than nHAC. All these results demonstrate the usefulness ofnanofiber-peptide composited nHAC scaffolds for inducing enhanced bone formation.In this way, the application of nanofiber-peptide to modify nHAC is an easy andefficient way to promote cell proliferation and osteogenic differentiation, and boneregeneration.