Establishment Of The Prostate Cancer Orthotopic Model In Mice And The Role Of Kng In Prostate Cancer

Part1Establishment of the prostate cancer orthotopic model in miceObjective To establish a prostate cancer orthotopic model in mice and discuss itsvalue of application.Methods Histologically intact tumor tissues harvested from a subcutaneous prostatetumor of C57BL6syngenic mouse were cut into small pieces and transplanted into micesubcutaneous （subcutaneous group,10） and prostate gland （orthotopic group,30）. The rateof metastasis and the survival time of these two models were compared.Results The rate of tumor formation of subcutaneous group and orthotopic group was100%. The survival time of orthotopic model was （14.6±2.6d） days,markedly shorter thanthat of subcutaneous model （39.8±4.7d） days（P<0.01）. The rate of metastasis of orthotopicgroup mice was more high than the mice of subcutaneous group （73.3%vs0, P <0.01）.Conclusion The prostate cancer orthotopic model is a stable, high rate of metastasisto distal organ model, it is an optimal clinical animal model to observe metastasis.Part2The role of Kng in prostate cancerObjective To explore the inhibitory effect of Kng on metastasis of prostate cancer.Methods The kininogen protein and kininogen mRNA in prostate cancer wereconfirmed by Western-blotting and RT-PCR. Using fifteen Kng^-/-mice and Kng^+/+mice, theprostate cancer orthotopic model were established. The rate of metastasis and the survivaltime of these two models were compared.Results The kininogen protein and kininogen mRNA in mice prostate cancer weremore high than mice prostate.In fifty days,12Kng^-/-mice were died（12/15） and15Kng^+/+mice were died（15/15）. The rate of metastasis of Kng^-/-mice was markedly lower than theKng^+/+mice（20%vs66.7%,P<0.05）.Conclusion1. The level of kininogen protein and kininogen mRNA is high in prostatecancer.2. The kininogen gene knockout can prolong survival time of prostate cancer.3.The kininogen gene knockout can reduce the rate of metastasis of prostate cancer.