Study On The Change Of NSE、S-100B And GFAP In The Blood Serum Of Immature Rats After Epileptic Seizure And Its Importance

Purpose:To observe the dynamic changes of NSE, S-100B and GFAP inthe blood serum of immature rats after epileptic seizure, and to discuss therole and significance of these biochemical indicators in curing the epilepticseizure.Method:To construct a neurons of epileptic rat model with SD rats of5days,and to induce those rats with flurothyl. Then divide those rats into3groups at random: normal control, single convulsion, and repeated convulsion.To determine NSE、S-100B、GFAP in the blood serum of rats in the3groupswith the method of DAS-ELISA, and separately determine the value of1dayafter epileptic seizure,2days after epileptic seizure,7days after epilepticseizure and15days after epileptic seizure.Result: After a single convulsion, NSE、S-100B in the serum of animmature rat has increased significantly at the time of1day. Then decreasedby degree at each time point and has no significant difference compared to thecontrol group. There is no significant difference in GFAP. After a repeatedconvulsion, it can be founded that NSE and S-100B in the blood serumincreased significantly at the time of1day and2days. At the time of1day,the values reached the top. The values of7days and15days have no significant difference compared to the control group. The values of GFAP areall above those of the control group. But significant differences only can befound in the values of7days compared to the control group.Conclusion: After a single convulsion, NSE、S-100B in the blood serumof an immature rat have increased at1st day, but no significant increase atother time points. NSE and S-100B have increased gradually and the increasewould last to the2ndday after the convulsion. NSE and S-100B are thesensitive biochemical markers of early stage brain lesions after the epilepticseizure. A single convulsion does lesser damage to the brain, but a repeatedconvulsion does longer damage to the brain. There is no significant differencein the GFAP after a single convulsion. It shows that GFAP is not the sensitivebiochemical marker of single convulsive brain lesions. GFAP has obviouslyincreased at the7thday after a repeated convulsion. It is even higher than thatof the control group after the15thday. It shows that the increase of GFAPmight be related to the brain lesion after the repeated convulsion and theincrease of GFAP itself.